Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations

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Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l(-1), cell growth and PHB synthesis were limited due to the shortage of carbon source but a higher PHB content occurred in the cell-growth stage. This shows that a low glucose concentration is favorable for PHB accumulation in R. eutropha. PHB obtained with glucose at 9 g l(-1) is 1.6 times that obtained with 40 g l(-1). When glucose was in the range of 9 to 40 g l(-1), PHB concentration and productivity decreased significantly with the increase of glucose concentration. The highest PHB productivity was obtained with glucose at 9 g l(-1).
Publisher
Springer
Issue Date
2003-09
Language
English
Article Type
Article
Keywords

ALCALIGENES-EUTROPHUS; ESCHERICHIA-COLI; PROPIONIC-ACID; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)

Citation

BIOTECHNOLOGY LETTERS, v.25, no.17, pp.1415 - 1419

ISSN
0141-5492
URI
http://hdl.handle.net/10203/82542
Appears in Collection
CBE-Journal Papers(저널논문)
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