DC Field | Value | Language |
---|---|---|
dc.contributor.author | Di Carlo, D. | ko |
dc.contributor.author | Jeong, KI-HUN | ko |
dc.contributor.author | Lee, L.P. | ko |
dc.date.accessioned | 2013-03-04T03:35:25Z | - |
dc.date.available | 2013-03-04T03:35:25Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | LAB ON A CHIP, v.3, no.4, pp.287 - 291 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/81751 | - |
dc.description.abstract | A highly effective, reagentless, mechanical cell lysis device integrated in microfluidic channels is reported. Sample preparation, specifically cell lysis, is a critical element in 'lab-on-chip' applications. However, traditional methods of cell lysis require purification steps or complicated fabrication steps that a simple mechanical method of lysis may avoid. A simple and effective mechanical cell lysis system is designed, microfabricated, and characterized to quantify the efficiency of cell lysis and biomolecule accessibility. The device functionality is based on a microfluidic filter region with nanostructured barbs created using a modified deep reactive ion etching process. Mechanical lysis is characterized by using a membrane impermeable dye. Three main mechanisms of micro-mechanical lysis are described. Quantitative measurements of accessible protein as compared to a chemically lysed sample are acquired with optical absorption measurements at 280 and 414 nm. At a flow rate of 300 muL min(-1) within the filter region total protein and hemoglobin accessibilities of 4.8% and 7.5% are observed respectively as compared to 1.9% and 3.2% for a filter without nanostructured barbs. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation | - |
dc.type | Article | - |
dc.identifier.wosid | 000186468400015 | - |
dc.identifier.scopusid | 2-s2.0-1842834076 | - |
dc.type.rims | ART | - |
dc.citation.volume | 3 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 287 | - |
dc.citation.endingpage | 291 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/b305162e | - |
dc.contributor.localauthor | Jeong, KI-HUN | - |
dc.contributor.nonIdAuthor | Di Carlo, D. | - |
dc.contributor.nonIdAuthor | Lee, L.P. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PCR AMPLIFICATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BLOOD-CELLS | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | LATTICE | - |
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