DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pawlak, Geraldine | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-03-04T03:16:41Z | - |
dc.date.available | 2013-03-04T03:16:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-07 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.277, no.30, pp.26927 - 26933 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10203/81692 | - |
dc.description.abstract | Cellular transformation by v-Src is believed to be caused by aberrant activation of signaling pathways that are normally regulated by cellular Src. Using normal rat kidney cells expressing a temperature-sensitive mutant of v-Src, we examined the role of the Raf/MEK/ERK, phosphatidylinositol 3-kinase/Akt, and Rho pathways in morphological transformation and cytoskeletal changes induced by v-Src. Activation of v-Src elicited a loss of actin stress fibers and focal contacts. A decrease in the phosphorylation level of cofilin was detected upon v-Src activation, which is indicative of attenuated Rho function. Inhibition of MEK using U0126 prevented v-Src-induced disruption of the cytoskeleton as well as dephosphorylation of cofilin, whereas treatment with a phosphatidylinositol 3-kinase inhibitor had no protective effect. In normal rat kidney cells stably transformed by v-Src, we found that the chronic activation of MEK induces down-regulation of ROCK expression, thereby uncoupling Rho from stress fiber formation. Taken together, these results establish MEK as an effector of v-Src-induced cytoskeleton disruption, participating in v-Src-induced antagonism of the cellular function of Rho. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | SMOOTH-MUSCLE CONTRACTION | - |
dc.subject | FOCAL ADHESIONS | - |
dc.subject | STRESS FIBERS | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | TRANSFORMATION | - |
dc.subject | PROTEIN | - |
dc.subject | REORGANIZATION | - |
dc.subject | CELLS | - |
dc.subject | COFILIN | - |
dc.subject | DYNAMICS | - |
dc.title | MEK mediates v-Src-induced disruption of the actin cytoskeleton via inactivation of the Rho-ROCK-LIM kinase pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000177055900031 | - |
dc.identifier.scopusid | 2-s2.0-0037178873 | - |
dc.type.rims | ART | - |
dc.citation.volume | 277 | - |
dc.citation.issue | 30 | - |
dc.citation.beginningpage | 26927 | - |
dc.citation.endingpage | 26933 | - |
dc.citation.publicationname | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.M202261200 | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Pawlak, Geraldine | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SMOOTH-MUSCLE CONTRACTION | - |
dc.subject.keywordPlus | FOCAL ADHESIONS | - |
dc.subject.keywordPlus | STRESS FIBERS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | REORGANIZATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | COFILIN | - |
dc.subject.keywordPlus | DYNAMICS | - |
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