DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho K.S. | ko |
dc.contributor.author | Lee J.H. | ko |
dc.contributor.author | Kim S. | ko |
dc.contributor.author | Kim D. | ko |
dc.contributor.author | Koh H. | ko |
dc.contributor.author | Lee J. | ko |
dc.contributor.author | Kim J. | ko |
dc.contributor.author | Chung, Jongkyeong | ko |
dc.contributor.author | Kim C. | ko |
dc.date.accessioned | 2013-03-03T23:42:52Z | - |
dc.date.available | 2013-03-03T23:42:52Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.98, no.11, pp.6144 - 6149 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/80952 | - |
dc.description.abstract | Phosphoinositide-dependent kinase-1 (PDK-1) is a central mediator of the cell signaling between phosphoinositide 3-kinase (PI3K) and various intracellular serine/threonine kinases including Akt/protein kinase B (PKB), p70 56 kinases, and protein kinase C, Recent studies with cell transfection experiments have implied that PDK-1 may be involved in various cell functions including cell growth and apoptosis, However, despite its pivotal role in cellular signalings, the in vivo functions of PDK-1 in a multicellular system have rarely been investigated. Here, we have isolated Drosophila PDK-1 (dPDK-1) mutants and characterized the in vivo roles of the kinase, Drosophila deficient in the dPDK-1 gene exhibited lethality and an apoptotic phenotype in the embryonic stage. Conversely, overexpression of dPDK-1 increased cell and organ size in a Drosophila PI3K-dependent manner. dPDK-1 not only could activate Drosophila Akt/PKB (Dakt1), but also substitute the in vivo functions of its mammalian ortholog to activate Akt/PKB, This functional interaction between dPDK-1 and Dakt1 was further confirmed through genetic analyses in Drosophila. On the other hand, cAMP-dependent protein kinase, which has been proposed as a possible target of dPDK- I, did not interact with dPDK-1. In conclusion, our findings provide direct evidence that dPDK-1 regulates cell growth and apoptosis during Drosophila development via the PI3K-dependent signaling pathway and demonstrate our Drosophila system to be a powerful tool for elucidating the in vivo functions and targets of PDK-1. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE-1 | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | S6 KINASE | - |
dc.subject | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject | CELL-DEATH | - |
dc.subject | AKT | - |
dc.subject | PDK1 | - |
dc.subject | ACTIVATION | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | GENE | - |
dc.title | Drosophila phosphoinositide-dependent kinase-1 regulates apoptosis and growth via the phosphoinositide 3-kinase-dependent signaling pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000168883700039 | - |
dc.identifier.scopusid | 2-s2.0-0007588936 | - |
dc.type.rims | ART | - |
dc.citation.volume | 98 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 6144 | - |
dc.citation.endingpage | 6149 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.101596998 | - |
dc.contributor.localauthor | Kim J. | - |
dc.contributor.localauthor | Chung, Jongkyeong | - |
dc.contributor.nonIdAuthor | Cho K.S. | - |
dc.contributor.nonIdAuthor | Lee J.H. | - |
dc.contributor.nonIdAuthor | Kim S. | - |
dc.contributor.nonIdAuthor | Kim D. | - |
dc.contributor.nonIdAuthor | Koh H. | - |
dc.contributor.nonIdAuthor | Lee J. | - |
dc.contributor.nonIdAuthor | Kim C. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE-1 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | S6 KINASE | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | AKT | - |
dc.subject.keywordPlus | PDK1 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | GENE | - |
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