DC Field | Value | Language |
---|---|---|
dc.contributor.author | Na, YJ | ko |
dc.contributor.author | Jeon, YJ | ko |
dc.contributor.author | Suh, JH | ko |
dc.contributor.author | Kang, JS | ko |
dc.contributor.author | Yang, Kyu-Hwan | ko |
dc.contributor.author | H. W.Kim | ko |
dc.date.accessioned | 2013-03-03T22:40:37Z | - |
dc.date.available | 2013-03-03T22:40:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-09 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, v.1, no.9-10, pp.1877 - 1887 | - |
dc.identifier.issn | 0192-0561 | - |
dc.identifier.uri | http://hdl.handle.net/10203/80760 | - |
dc.description.abstract | We show that radicicol, an anti-fungal agent, inhibits interleukin-8 (IL-8) production by the human monocyte line THP-1 in response to phorbol-12-myristate-13- acetate/lipopolysaccharide (PMA/LPS). IL-8 is a potent chemokine and needs for an optimal immune response-such as inflammation by activation of neutrophils. The decrease in PMA/LPS-induced IL-8 mRNA expression was demonstrated by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Since the promoter in IL-8 gene contains binding motifs for NF-kappaB, AP-1, and NF-IL-6, which appear to be important in IL-8 induction, the effects of radicicol on the activation of these transcription factors were examined. Treatment of radicicol to THP-I cells produced a strong inhibition of NF-kappaB and AP-1, while NF-IL6 was not significantly affected by radicicol. Western blot analysis showed that radicicol inhibited the phosphorylation and phosphotransferase activities of extracellular signal-regulated kinases I and 2 (ERK1/2) and p38. PD98059 and SB203580, known as a specific inhibitor of MEK1 and p38 kinase, respectively, inhibited IL-8 gene expression showing that both of the kinase pathways are involved in IL-8 regulation in human monocytes, Collectively, this series of experiments indicates that radicicol inhibits IL-8 gene expression by blocking ERK1/2 and p38 signaling. (C) 2001 Elsevier Science BN. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Suppression of IL-8 gene expression by radicicol is mediated through the inhibition of ERK1/2 and p38 signaling and negative regulation of NF-kappa B and AP-1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000170818500023 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1 | - |
dc.citation.issue | 9-10 | - |
dc.citation.beginningpage | 1877 | - |
dc.citation.endingpage | 1887 | - |
dc.citation.publicationname | INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY | - |
dc.identifier.doi | 10.1016/S1567-5769(01)00113-8 | - |
dc.contributor.nonIdAuthor | Na, YJ | - |
dc.contributor.nonIdAuthor | Jeon, YJ | - |
dc.contributor.nonIdAuthor | Suh, JH | - |
dc.contributor.nonIdAuthor | Kang, JS | - |
dc.contributor.nonIdAuthor | H. W.Kim | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | radicicol | - |
dc.subject.keywordAuthor | interleukin-8 | - |
dc.subject.keywordAuthor | ERK1/2 | - |
dc.subject.keywordAuthor | p38 | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | AP-1 | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | INTERLEUKIN-8 GENE | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | MONOCYTIC CELLS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | NEUTROPHIL | - |
dc.subject.keywordPlus | CYTOKINES | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.