DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim D. | ko |
dc.contributor.author | Kim S. | ko |
dc.contributor.author | Yoon S.-O. | ko |
dc.contributor.author | Chung A.-S. | ko |
dc.contributor.author | Cho K.S. | ko |
dc.contributor.author | Chung, Jongkyeong | ko |
dc.contributor.author | Koh H | ko |
dc.date.accessioned | 2013-03-03T22:07:20Z | - |
dc.date.available | 2013-03-03T22:07:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | FASEB JOURNAL, v.15, no.11, pp.1953 - 1962 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10203/80655 | - |
dc.description.abstract | The Akt/protein kinase B (PKB) serine/threonine kinase is well known as an important mediator of many cell survival signaling pathways. Here, we demonstrate for the first time a major role of Akt/PKB in the cell invasion properties of the highly metastatic cell line HT1080. Using confocal microscopic analyses of live samples, we found Akt/PKB to be localized in the leading edge membrane area of migrating HT1080 cells. This localization was dependent on phosphoinositide 3-kinase and required the lipid binding ability of the phosphoinositide binding pleckstrin homology domain of Akt/PKB. We examined the possible function of Akt/PKB in HT1080 invasion. Surprisingly, Akt/PKB potently promoted HT1080 invasion, by increasing cell motility and matrix metalloproteinase-9 (MMP-9) production, in a manner highly dependent on its kinase activity and membrane-translocating ability. The increase in MMP-9 production was mediated by activation of nuclear factor-kappaB transcriptional activity by Akt/PKB. However, Akt/PKB did not affect the cell-cell or cell-matrix adhesion properties of HT1080. Our findings thus establish Akt/PKB as a major factor in the invasive abilities of cancer cells. | - |
dc.language | English | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | PROTEIN-KINASE-B | - |
dc.subject | AKT PROTOONCOGENE | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | ACTIN ORGANIZATION | - |
dc.subject | SURVIVAL SIGNALS | - |
dc.subject | IV COLLAGENASE | - |
dc.subject | METASTASIS | - |
dc.subject | ACTIVATION | - |
dc.subject | RAC | - |
dc.title | Akt/PKB promotes cancer cell invasion via increased motility and metalloproteinase production | - |
dc.type | Article | - |
dc.identifier.wosid | 000171372700010 | - |
dc.identifier.scopusid | 2-s2.0-0034852195 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 1953 | - |
dc.citation.endingpage | 1962 | - |
dc.citation.publicationname | FASEB JOURNAL | - |
dc.identifier.doi | 10.1096/fj.01-0198com | - |
dc.contributor.localauthor | Chung, Jongkyeong | - |
dc.contributor.nonIdAuthor | Kim D. | - |
dc.contributor.nonIdAuthor | Kim S. | - |
dc.contributor.nonIdAuthor | Yoon S.-O. | - |
dc.contributor.nonIdAuthor | Chung A.-S. | - |
dc.contributor.nonIdAuthor | Cho K.S. | - |
dc.contributor.nonIdAuthor | Koh H | - |
dc.type.journalArticle | Article | - |
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