Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate

Cited 33 time in webofscience Cited 0 time in scopus
  • Hit : 272
  • Download : 0
A simple and effective method for the recovery poly(3-hydroxybutyrate) [P(3HB)] directly from high cell density culture broth with no pretreatment steps has been developed. This method consists of direct addition of sodium dodecyl sulfate (SDS) to the culture broth, shaking, heat treatment, and washing steps. When the SDS/biomass ratio was higher than 0.4, the purity of recovered P(3HB) was over 95% for various cell concentrations of 50-300 g dry cell l(-1), with the highest value of 97%. The recovery of P(3HB) was over 90% regardless of cell concentration and SDS dosage (SDS/biomass ratios, 0.1-0.7). One g SDS digests 0.72 g non-P(3HB) cell materials. The reduction in molecular weight, due to degradation of P( 3HB) by SDS, was negligible.
Publisher
KLUWER ACADEMIC PUBL
Issue Date
2003-01
Language
English
Article Type
Article
Keywords

ALCALIGENES-EUTROPHUS; ESCHERICHIA-COLI

Citation

BIOTECHNOLOGY LETTERS, v.25, no.1, pp.55 - 59

ISSN
0141-5492
URI
http://hdl.handle.net/10203/80370
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 33 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0