DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, JH | ko |
dc.contributor.author | Ryu, GH | ko |
dc.contributor.author | Finch, P | ko |
dc.contributor.author | Rhee, Joon Shick | ko |
dc.date.accessioned | 2013-03-03T20:27:04Z | - |
dc.date.available | 2013-03-03T20:27:04Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-11 | - |
dc.identifier.citation | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.15, no.4-6, pp.191 - 196 | - |
dc.identifier.issn | 1381-1177 | - |
dc.identifier.uri | http://hdl.handle.net/10203/80296 | - |
dc.description.abstract | Enzymatic synthesis of 3-O-methyl-4-O-beta -D-galactopyranosyl-D-glucose (3-O-methyl-lactose) has been attempted using both galactosyltransferase and galactosidase activities. The transferase-catalysed reaction produces exclusively the desired product in beta -1,4-glycosidic linkage whereas the galactosidase-catalysed reactions predominantly form a 1,6-linked disaccharide. With galactosidase, in order to change the regioselectivity, blocking of the 6-position of 3-O-methyl-D-glucose and anomeric modification of the acceptor structure were investigated. Although acetylation of the 6-position of 3-O-methyl glucose catalysed by lipase was successful, the synthesis of the desired disaccharide did not occur. (C) 2001 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | SUBSTRATE-SPECIFICITY | - |
dc.subject | ACCEPTOR SPECIFICITY | - |
dc.subject | BETA-GALACTOSIDASES | - |
dc.subject | N-ACETYLLACTOSAMINE | - |
dc.subject | LACTOSE HYDROLYSIS | - |
dc.subject | ENZYMIC-SYNTHESIS | - |
dc.subject | GLYCOSIDES | - |
dc.subject | DISACCHARIDES | - |
dc.subject | HYDROGEN | - |
dc.title | Enzymatic synthesis of 3-O-methyl-4-O-beta-D-galactopyranosyl-D-glucose (3-O-methyl-lactose); a potential agent for the assessment of intestinal lactase activity | - |
dc.type | Article | - |
dc.identifier.wosid | 000171658200010 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 4-6 | - |
dc.citation.beginningpage | 191 | - |
dc.citation.endingpage | 196 | - |
dc.citation.publicationname | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | - |
dc.identifier.doi | 10.1016/S1381-1177(01)00028-5 | - |
dc.contributor.nonIdAuthor | Yoon, JH | - |
dc.contributor.nonIdAuthor | Ryu, GH | - |
dc.contributor.nonIdAuthor | Finch, P | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | lactasia | - |
dc.subject.keywordAuthor | enzymatic synthesis | - |
dc.subject.keywordAuthor | galactosyltransferase | - |
dc.subject.keywordAuthor | beta-galactosidase | - |
dc.subject.keywordAuthor | 3-O-methyl-lactose | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | ACCEPTOR SPECIFICITY | - |
dc.subject.keywordPlus | BETA-GALACTOSIDASES | - |
dc.subject.keywordPlus | N-ACETYLLACTOSAMINE | - |
dc.subject.keywordPlus | LACTOSE HYDROLYSIS | - |
dc.subject.keywordPlus | ENZYMIC-SYNTHESIS | - |
dc.subject.keywordPlus | GLYCOSIDES | - |
dc.subject.keywordPlus | DISACCHARIDES | - |
dc.subject.keywordPlus | HYDROGEN | - |
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