The feeding method of propionic acid for production of poly(3-hydroxybutyrate-co-3-hydro xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstonia eutropha was optimized to achieve high cell density and high 3HV yield. Effects of different feeding strategies of propionic acid on the production of P(3HB-co-3HV) were investigated. A decline of specific synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due to the propionic acid accumulation in culture broth when the feeding solution with high P/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling propionic acid concentration at a low level in the separate feeding of propionic acid. An optimal feeding strategy was demonstrated to reduce the propionic acid accumulation. The cell concentration, P (3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg(.)m(-3), 1.8kg(.)m(-3.)h(-1), and 10.6%(by mass), respectively, resulting in a yield of 0.33g HV per g propionic acid.