DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Y | ko |
dc.contributor.author | Park, C | ko |
dc.date.accessioned | 2013-03-03T08:21:34Z | - |
dc.date.available | 2013-03-03T08:21:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-02 | - |
dc.identifier.citation | JOURNAL OF BACTERIOLOGY, v.181, no.3, pp.1039 - 1042 | - |
dc.identifier.issn | 0021-9193 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77956 | - |
dc.description.abstract | RbsC of Escherichia coli is the hydrophobic membrane component of ribose uptake system classified as the ATP-binding cassette transporter. To understand the structure and function of RbsC, its transmembrane topology was investigated by using 64 RbsC-PhoA fusions isolated either specifically or randomly. In order to confirm the cytoplasmic location of the short C-terminal region (5 amino acids), inside-out or right-side-out membrane vesicles were generated, and the C-terminal region was found to be digested by carboxypeptidase A only in inside-out vesicles. This result is consistent with the model, based on the results of alkaline phosphatase fusions, in which the protein traverses the membrane six times and the N and C termini are exposed to the cytoplasm. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | ESCHERICHIA-COLI K-12 | - |
dc.subject | BINDING-PROTEIN | - |
dc.subject | ALKALINE-PHOSPHATASE | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | PERMEASE | - |
dc.subject | FUSIONS | - |
dc.subject | OPERON | - |
dc.subject | SYSTEM | - |
dc.subject | DETERMINANTS | - |
dc.subject | RESIDUES | - |
dc.title | Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily | - |
dc.type | Article | - |
dc.identifier.wosid | 000078390600045 | - |
dc.identifier.scopusid | 2-s2.0-0032890882 | - |
dc.type.rims | ART | - |
dc.citation.volume | 181 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1039 | - |
dc.citation.endingpage | 1042 | - |
dc.citation.publicationname | JOURNAL OF BACTERIOLOGY | - |
dc.contributor.localauthor | Park, C | - |
dc.contributor.nonIdAuthor | Park, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI K-12 | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | ALKALINE-PHOSPHATASE | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | PERMEASE | - |
dc.subject.keywordPlus | FUSIONS | - |
dc.subject.keywordPlus | OPERON | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | DETERMINANTS | - |
dc.subject.keywordPlus | RESIDUES | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.