DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hail | ko |
dc.contributor.author | Kim, J | ko |
dc.contributor.author | Kim, S | ko |
dc.contributor.author | Cha, JY | ko |
dc.contributor.author | Kim, KS | ko |
dc.contributor.author | Ahn, Y | ko |
dc.date.accessioned | 2013-03-03T07:36:42Z | - |
dc.date.available | 2013-03-03T07:36:42Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-09 | - |
dc.identifier.citation | DIABETES, v.49, no.9, pp.1517 - 1524 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77800 | - |
dc.description.abstract | We identified the peroxisomal proliferator response element (PPRE) in the +68/+89 region of the rat GLUT2 gene. To identify whether the putative PPRE in the GLUTS gene (GLUT2-PPRE) is functional, GLUTS promoter-luciferase reporter constructs were transfected into CV-1 cells. Promoter activities were increased by coexpression of peroxisomal proliferator-activated receptor (PPAR)-gamma, retinoid X receptor (RXR)-alpha, and treatment of their ligands; troglitazone and g-cis retinoic acid potentiated the transactivational effects, Introduction of mutations in GLUT2-PPBE resulted in loss of transactivational effects of the PPAR-gamma/RXR-alpha heterodimer, Electrophoretic mobility shift assay using nuclear extracts of CV-1 cells, which were transfected with various combinations of PPARs or RXR-alpha expression plasmids, revealed that heterodimers of PPAR-gamma and RXR-alpha preferentially bound to GLUT2-PPRE. In HIT-T15 cells, promoter activity of the mt GLUTS gene was increased by troglitazone and g-cis retinoic acid, and mutations of GLUT2-PPRE resulted in reduction of promoter activity. In addition, we observed increased GLUTS transcription by troglitazone and 9-cis retinoic acid in isolated rat primary islets, These results suggested that the GLUT2-PPRE is functional and plays a significant role in gene expression of GLUTS in pancreatic beta-cells. This is the first report; identifying PPRE in a gene involved in glucose homeostasis, linking the effect; of troglitazone on the regulation of insulin secretion. | - |
dc.language | English | - |
dc.publisher | AMER DIABETES ASSOC | - |
dc.subject | GLUCOSE-TRANSPORTER GENE | - |
dc.subject | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject | PPAR-GAMMA | - |
dc.subject | TROGLITAZONE CS-045 | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | FATTY RATS | - |
dc.subject | EXPRESSION | - |
dc.subject | BINDING | - |
dc.subject | CELLS | - |
dc.subject | ACID | - |
dc.title | Identification and functional characterization of the peroxisomal proliferator response element in rat GLUT2 promoter | - |
dc.type | Article | - |
dc.identifier.wosid | 000089062800016 | - |
dc.identifier.scopusid | 2-s2.0-0033864402 | - |
dc.type.rims | ART | - |
dc.citation.volume | 49 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 1517 | - |
dc.citation.endingpage | 1524 | - |
dc.citation.publicationname | DIABETES | - |
dc.identifier.doi | 10.2337/diabetes.49.9.1517 | - |
dc.contributor.localauthor | Kim, Hail | - |
dc.contributor.nonIdAuthor | Kim, J | - |
dc.contributor.nonIdAuthor | Kim, S | - |
dc.contributor.nonIdAuthor | Cha, JY | - |
dc.contributor.nonIdAuthor | Kim, KS | - |
dc.contributor.nonIdAuthor | Ahn, Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GLUCOSE-TRANSPORTER GENE | - |
dc.subject.keywordPlus | ACTIVATED RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | TROGLITAZONE CS-045 | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | FATTY RATS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ACID | - |
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