DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, W | ko |
dc.contributor.author | KANG, Changwon | ko |
dc.date.accessioned | 2013-03-03T07:36:01Z | - |
dc.date.available | 2013-03-03T07:36:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997-07 | - |
dc.identifier.citation | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, v.42, no.4, pp.711 - 716 | - |
dc.identifier.issn | 1039-9712 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77798 | - |
dc.description.abstract | In order to identify functional residues of the bacteriophage SP6 RNA polymerase, its C-terminal one-twelfth region was randomly mutagenized using polymerase chain reactions of its gene under the conditions for reduced fidelity of Taq DNA polymerase. Using a two-vector system that permits phenotypic isolation of mutants with reduced in vivo transcription activity, 3 single and 1 multiple mutants were isolated. A single substitution of Gln for His805 resulted in complete inactivation of the enzyme. A multiple mutant carrying substitutions at 808, 820, 835, 843 and 848 also abolished the activity. However, changes of Pro856-->Ser and Asp862-->Glu individually reduced the activity only slightly. It is noteworthy that His805 is one of the two motif-C residues that are absolutely conserved among all the DNA polymerases and monomeric RNA polymerases. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS AUST | - |
dc.subject | DNA-POLYMERASE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | FRAGMENT | - |
dc.title | The Histidine-805 in motif-C of the phage SP6 RNA polymerase is essential for its activity as revealed by random mutagenesis | - |
dc.type | Article | - |
dc.identifier.wosid | 000072915200008 | - |
dc.identifier.scopusid | 2-s2.0-0031398382 | - |
dc.type.rims | ART | - |
dc.citation.volume | 42 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 711 | - |
dc.citation.endingpage | 716 | - |
dc.citation.publicationname | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | - |
dc.contributor.localauthor | KANG, Changwon | - |
dc.contributor.nonIdAuthor | Jeong, W | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DNA-POLYMERASE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FRAGMENT | - |
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