A bacterial strain, LE31(T), which is capable of degrading 3-ethylpyridine and 3-methylpyridine, was isolated from an industrial wastewater and was taxonomically studied by using a polyphasic approach. Strain LE31(T) was identified as a member of the genus Gordonia on the basis of chemotaxonomic characteristics and phylogenetic inference-based 16S rDNA sequence. The cell wall contained meso-diaminopimelic acid, arabinose and galactose (wall chemotype IV). The predominant menaquinone was MK-9(H-2). The mycolic acids contained 47-55 carbon atoms. The major fatty acids were C-16:0, C-18:1 omega 9c, 10-methyl-C-18:0 (TBSA). The G+C content of DNA was 67 mol%. The 16S rDNA sequence of strain LE31(T) was most similar to that of the type strain of Gordonia rubropertincta. The differences in some phenotypic characteristics and the genetic distinctiveness distinguish strain LE31(T) from the Gordonia species described previously. Therefore it is proposed that strain LE31(T) should be placed in the genus Gordonii, as a new species. The name Gordonia nitida is proposed for strain LE31(T). The type strain of the new species is strain LE31(T) (= KCTC 0605BP(T) = KCCM 80004(T)).