DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Day, TA | ko |
dc.contributor.author | Bennett, JL | ko |
dc.contributor.author | Pax, RA | ko |
dc.date.accessioned | 2013-03-03T06:54:21Z | - |
dc.date.available | 2013-03-03T06:54:21Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-02 | - |
dc.identifier.citation | PARASITOLOGY, v.110, pp.180 - 2 | - |
dc.identifier.issn | 0031-1820 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77692 | - |
dc.description.abstract | We have isolated a cDNA (SKv1.1) encoding a Shaker-related K+ channel from an adult cDNA library of the human parasitic trematode Schistosoma mansoni. The deduced amino acid sequence (512 aa, 56.5 kDa) contains 6 putative membrane-spanning domains (S1-S6) and a pore-forming domain (H5). SKv1.1 is grouped in the Shaker family, but forms a unique branch within this family, on the basis of dendrogram analysis. SKv1.1 shows significant sequence identity with most other Shaker channels, with 64-74% identity in the core region (S1-S6). It has the highest sequence identity with the K+ channel (Ak01a) from Aplysia. Northern blot analysis detected a single primary transcript of 2.8 kb. Southern blot analysis indicated that SKv1.1 is present as a single copy in the genomic DNA of S. mansoni. Expression of SKv1.1 in Xenopus oocytes produced a rapidly activating and inactivating outward K+ current which is highly sensitive to 4-aminopyridine, but is insensitive to tetraethylammonium, mast cell degranulating peptide, dendrotoxin and charybdotoxin. The presence of a Shaker homologue in Schistosoma suggests that Sh subfamilies may exist in other lower invertebrates as well as platyhelminths. | - |
dc.language | English | - |
dc.publisher | CAMBRIDGE UNIV PRESS | - |
dc.subject | RECTIFIER K+ CHANNEL | - |
dc.subject | XENOPUS OOCYTES | - |
dc.subject | RAT-BRAIN | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | DROSOPHILA | - |
dc.subject | DIVERSITY | - |
dc.subject | CURRENTS | - |
dc.subject | MULTIPLE | - |
dc.subject | RECEPTOR | - |
dc.subject | FAMILY | - |
dc.title | Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda: Digenea) | - |
dc.type | Article | - |
dc.identifier.wosid | A1995QG60100008 | - |
dc.identifier.scopusid | 2-s2.0-0028861603 | - |
dc.type.rims | ART | - |
dc.citation.volume | 110 | - |
dc.citation.beginningpage | 180 | - |
dc.citation.endingpage | 2 | - |
dc.citation.publicationname | PARASITOLOGY | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Day, TA | - |
dc.contributor.nonIdAuthor | Bennett, JL | - |
dc.contributor.nonIdAuthor | Pax, RA | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | SCHISTOSOMA MANSONI | - |
dc.subject.keywordAuthor | PLATYHELMINTH | - |
dc.subject.keywordAuthor | TREMATODE | - |
dc.subject.keywordAuthor | POTASSIUM CHANNEL | - |
dc.subject.keywordAuthor | SHAKER | - |
dc.subject.keywordAuthor | CLONING | - |
dc.subject.keywordAuthor | EXPRESSION | - |
dc.subject.keywordAuthor | XENOPUS | - |
dc.subject.keywordAuthor | OOCYTE | - |
dc.subject.keywordPlus | RECTIFIER K+ CHANNEL | - |
dc.subject.keywordPlus | XENOPUS OOCYTES | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | DROSOPHILA | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | MULTIPLE | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | FAMILY | - |
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