DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, EJ | ko |
dc.contributor.author | Kim, NS | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2013-03-03T06:44:20Z | - |
dc.date.available | 2013-03-03T06:44:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-04 | - |
dc.identifier.citation | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, v.35, no.4, pp.178 - 182 | - |
dc.identifier.issn | 1071-2690 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77663 | - |
dc.description.abstract | To develop serum-free (SF) medium for dihydrofolate reductase-deficient Chinese hamster ovary cells (DG44), a statistical optimization approach based on a Plackett-Burman design was adopted. DG44 cells which were normally maintained in 10% serum medium were gradually weaned to 0.5% serum medium to increase the probability of successful growth in SF medium. A basal medium was prepared by supplementing Dulbeccos modified Eagles medium and Hams nutrient mixture F12 with hypoxanthine (10 mg/l) and thymidine (10 mg/l). Twenty-eight different supplements were selected as variables on the basis of their growth-promoting abilities. From statistical analysis, leucine, tryptophan, lysine, proline, histidine, hydrocortisone, ethanolamine, and phosphatidylcholine were identified as important components showing positive effects on cell growth. A new SF medium (SF-DG44) was formulated by supplementing the basal medium with these components. When the weaned cells were inoculated at 1.0 x 10 cells/ml, a maximum viable cell concentration of 6.4 X 10 cells/ml was achieved in SF-DG44 medium. In contrast, when the unweaned cells were used, a concentration of only 4.1 X 10 cells/ml was reached under the same culture conditions, indicating that weaning of cells improves cell growth in SF medium. In summary, we found that development of a novel SF medium for DG44 cells was facilitated using a Plackett-Burman design technique and weaning of cells. | - |
dc.language | English | - |
dc.publisher | Springer New York | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CULTURE-MEDIUM | - |
dc.subject | GROWTH | - |
dc.subject | GENES | - |
dc.title | Development of a serum-free medium for dihydrofolate reductase-deficient Chinese hamster ovary cells (DG44) using a statistical design: Beneficial effect of weaning of cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000079708400002 | - |
dc.identifier.scopusid | 2-s2.0-0345435933 | - |
dc.type.rims | ART | - |
dc.citation.volume | 35 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 178 | - |
dc.citation.endingpage | 182 | - |
dc.citation.publicationname | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Kim, EJ | - |
dc.contributor.nonIdAuthor | Kim, NS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Plackett-Burman design | - |
dc.subject.keywordAuthor | serum-free medium | - |
dc.subject.keywordAuthor | DC44 cells | - |
dc.subject.keywordAuthor | weaning | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CULTURE-MEDIUM | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | GENES | - |
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