DC Field | Value | Language |
---|---|---|
dc.contributor.author | YOO, BJ | ko |
dc.contributor.author | SELBY, MJ | ko |
dc.contributor.author | Choe, Joonho | ko |
dc.contributor.author | SUH, BS | ko |
dc.contributor.author | CHOI, SH | ko |
dc.contributor.author | JOH, JS | ko |
dc.contributor.author | NUOVO, GJ | ko |
dc.contributor.author | LEE, HS | ko |
dc.contributor.author | HOUGHTON, M | ko |
dc.contributor.author | HAN, JH | ko |
dc.date.accessioned | 2013-03-03T06:43:29Z | - |
dc.date.available | 2013-03-03T06:43:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-01 | - |
dc.identifier.citation | JOURNAL OF VIROLOGY, v.69, no.1, pp.32 - 38 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77662 | - |
dc.description.abstract | T7 RNA polymerase transcripts of a putative full-length cDNA clone of hepatitis C virus type 1 (HCV-1) were used to transfect a differentiated human hepatoma cell line, Huh7. The transfected genome replicated in cells, as evidenced by the appearance of progeny HCV RNA, detection of negative-strand viral RNA, and incorporation of [H-3]uridine into the viral genome. Incubation of naive Huh7 cells with conditioned medium from transfected cells resulted in a new HCV infection, suggesting the production of biologically active virus in the inoculum. Maintenance of the transfected cells under serum-free culture conditions resulted in the selection of persistently infected cells which displayed a distinctive cellular morphology. This is the first demonstration that HCV RNA produced from cloned HCV cDNA is infectious and replication competent. This approach should provide a valuable system for studying HCV replication, persistence, and pathogenicity. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | NON-B-HEPATITIS | - |
dc.subject | 5&apos | - |
dc.subject | UNTRANSLATED REGION | - |
dc.subject | NON-A | - |
dc.subject | VIRAL-RNA | - |
dc.subject | 3&apos | - |
dc.subject | END | - |
dc.subject | GENOME | - |
dc.subject | CDNA | - |
dc.subject | IDENTIFICATION | - |
dc.subject | ORGANIZATION | - |
dc.subject | LOCALIZATION | - |
dc.title | Transfection of a differentiated human hepatoma cell line (Huh7) with in vitro-transcribed hepatitis C virus (HCV) RNA and establishment of a long-term culture persistently infected with HCV | - |
dc.type | Article | - |
dc.identifier.wosid | A1995PW81500004 | - |
dc.identifier.scopusid | 2-s2.0-0028961384 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 32 | - |
dc.citation.endingpage | 38 | - |
dc.citation.publicationname | JOURNAL OF VIROLOGY | - |
dc.contributor.localauthor | Choe, Joonho | - |
dc.contributor.nonIdAuthor | YOO, BJ | - |
dc.contributor.nonIdAuthor | SELBY, MJ | - |
dc.contributor.nonIdAuthor | SUH, BS | - |
dc.contributor.nonIdAuthor | CHOI, SH | - |
dc.contributor.nonIdAuthor | JOH, JS | - |
dc.contributor.nonIdAuthor | NUOVO, GJ | - |
dc.contributor.nonIdAuthor | LEE, HS | - |
dc.contributor.nonIdAuthor | HOUGHTON, M | - |
dc.contributor.nonIdAuthor | HAN, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NON-B-HEPATITIS | - |
dc.subject.keywordPlus | 5&apos | - |
dc.subject.keywordPlus | UNTRANSLATED REGION | - |
dc.subject.keywordPlus | NON-A | - |
dc.subject.keywordPlus | VIRAL-RNA | - |
dc.subject.keywordPlus | 3&apos | - |
dc.subject.keywordPlus | END | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | CDNA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ORGANIZATION | - |
dc.subject.keywordPlus | LOCALIZATION | - |
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