DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rao, A | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Sheng, M | ko |
dc.contributor.author | Craig, AM | ko |
dc.date.accessioned | 2013-03-03T06:25:35Z | - |
dc.date.available | 2013-03-03T06:25:35Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-02 | - |
dc.identifier.citation | JOURNAL OF NEUROSCIENCE, v.18, no.4, pp.1217 - 1229 | - |
dc.identifier.issn | 0270-6474 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77627 | - |
dc.description.abstract | To determine their roles in the assembly of glutamatergic postsynaptic sites, we studied the distributions of NMDA-and AMPA-type glutamate receptors; the NMDA receptor-interacting proteins alpha-actinin-2, PSD-95, and chapsyn; and the PSD-95-associated protein GKAP during the development of hippocampal neurons in culture. NMDA receptors first formed nonsynaptic proximal dendrite shaft clusters within 2-5 d. AMPA receptors were diffuse at this stage and began to cluster on spines at 9-10 d. NMDA receptor clusters remained partially nonsynaptic and mainly distinct from AMPA receptor clusters until after 3 weeks in culture, when the two began to colocalize at spiny synaptic sites. Thus, the localization of NMDA and AMPA receptors must be regulated by different mechanisms. alpha-Actinin-2 colocalized with the NMDA receptor only at spiny synaptic clusters, but not at shaft nonsynaptic or synaptic clusters, suggesting a modulatory role in the anchoring of NMDA receptor at spines. PSD-95, chapsyn, and GKAP were present at some, but not all, nonsynaptic NMDA receptor clusters during the first 2 weeks, indicating that none is essential for NMDA receptor cluster formation. When NMDA receptor clusters became synaptic, PSD-95 and GKAP were always present, consistent with an essential function in synaptic localization of NMDA receptors. Furthermore, PSD-95 and GKAP clustered opposite presynaptic terminals several days before either NMDA or AMPA receptors clustered at these presumptive postsynaptic sites. These results suggest that synapse development proceeds by formation of a postsynaptic scaffold containing PSD-95 and GKAP in concert with presynaptic vesicle clustering, followed by regulated attachment of glutamate receptor subtypes to this scaffold. | - |
dc.language | English | - |
dc.publisher | SOC NEUROSCIENCE | - |
dc.subject | NMDA RECEPTOR SUBUNITS | - |
dc.subject | RAT-BRAIN | - |
dc.subject | GLUTAMATE RECEPTORS | - |
dc.subject | MONKEY HIPPOCAMPUS | - |
dc.subject | GUANYLATE KINASES | - |
dc.subject | ENDOCRINE-CELLS | - |
dc.subject | SILENT SYNAPSES | - |
dc.subject | VISUAL-CORTEX | - |
dc.subject | PSD-95 FAMILY | - |
dc.subject | NR1 SUBUNIT | - |
dc.title | Heterogeneity in the molecular composition of excitatory postsynaptic sites during development of hippocampal neurons in culture | - |
dc.type | Article | - |
dc.identifier.wosid | 000071825800004 | - |
dc.identifier.scopusid | 2-s2.0-0032520191 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 1217 | - |
dc.citation.endingpage | 1229 | - |
dc.citation.publicationname | JOURNAL OF NEUROSCIENCE | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Rao, A | - |
dc.contributor.nonIdAuthor | Sheng, M | - |
dc.contributor.nonIdAuthor | Craig, AM | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | NMDA receptor | - |
dc.subject.keywordAuthor | AMPA receptor | - |
dc.subject.keywordAuthor | PSD-95 | - |
dc.subject.keywordAuthor | PSD-93 | - |
dc.subject.keywordAuthor | PDZ domain | - |
dc.subject.keywordAuthor | alpha-actinin-2 | - |
dc.subject.keywordAuthor | chapsyn | - |
dc.subject.keywordAuthor | GKAP | - |
dc.subject.keywordAuthor | postsynaptic membrane | - |
dc.subject.keywordAuthor | dendritic spines | - |
dc.subject.keywordPlus | NMDA RECEPTOR SUBUNITS | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | GLUTAMATE RECEPTORS | - |
dc.subject.keywordPlus | MONKEY HIPPOCAMPUS | - |
dc.subject.keywordPlus | GUANYLATE KINASES | - |
dc.subject.keywordPlus | ENDOCRINE-CELLS | - |
dc.subject.keywordPlus | SILENT SYNAPSES | - |
dc.subject.keywordPlus | VISUAL-CORTEX | - |
dc.subject.keywordPlus | PSD-95 FAMILY | - |
dc.subject.keywordPlus | NR1 SUBUNIT | - |
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