DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, DJ | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Francis, K. | ko |
dc.contributor.author | Palsson, BO | ko |
dc.date.accessioned | 2013-03-03T06:13:17Z | - |
dc.date.available | 2013-03-03T06:13:17Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-08 | - |
dc.identifier.citation | CYTOMETRY, v.36, no.4, pp.312 - 318 | - |
dc.identifier.issn | 0196-4763 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77581 | - |
dc.description.abstract | Background: The phototoxic effects of the well-known fluorescent membrane dyes PKH2 and PKH26 have been unknown, although their use in cell tracking experiments has increased dramatically. To eliminate the phototoxicity-induced alteration in cell function and morphology, it is essential to examine the suspicious phototoxicity of these dyes. Methods: Chemical and phototoxic effects of PKH dyes on the human hematopoietic KG1a cell line were examined. To minimize phototoxicity in long-term cell tracking experiments lasting up to 18 h with a fluorescence microscope system, time- lapse monitoring with different time intervals and exposure times was introduced. Results: There were no significant effects of the two PKH dyes on cell viability and growth when using dye concentrations up to 5 μM. However, when stained cells were exposed to excitation light, cell viability decreased dramatically, showing the phototoxicity of the PKH dyes. More than 60% of cells stained with 5 μM PKH26 died after 5 min of continuous light exposure. The phototoxic effect was more extensive in cells stained with higher concentrations of the dyes. Conclusions: We present guidelines for the optimal use of these dyes by using a defined hardware configuration. | - |
dc.language | English | - |
dc.publisher | John Wiley and Sons Inc. | - |
dc.subject | LYMPHOBLASTIC LEUKEMIC-CELLS | - |
dc.subject | MARROW STROMAL CELLS | - |
dc.subject | HAMSTER-CHEEK POUCH | - |
dc.subject | IN-VITRO | - |
dc.subject | PLATELET-AGGREGATION | - |
dc.subject | GROWTH-PARAMETERS | - |
dc.subject | INVIVO TRACKING | - |
dc.subject | VIVO | - |
dc.subject | SURVIVAL | - |
dc.subject | ADHESION | - |
dc.title | Phototoxicity of the fluorescent membrane dyes PKH2 and PKH26 on the human hematopoietic KG1a progenitor cell line | - |
dc.type | Article | - |
dc.identifier.wosid | 000081578600005 | - |
dc.identifier.scopusid | 2-s2.0-0344848624 | - |
dc.type.rims | ART | - |
dc.citation.volume | 36 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 312 | - |
dc.citation.endingpage | 318 | - |
dc.citation.publicationname | CYTOMETRY | - |
dc.identifier.doi | 10.1002/(SICI)1097-0320(19990801)36:4<312::AID-CYTO5>3.0.CO;2-V | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Oh, DJ | - |
dc.contributor.nonIdAuthor | Francis, K. | - |
dc.contributor.nonIdAuthor | Palsson, BO | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | phototoxicity | - |
dc.subject.keywordAuthor | PKH2 | - |
dc.subject.keywordAuthor | PKH26 | - |
dc.subject.keywordAuthor | fluorescence microscopy | - |
dc.subject.keywordAuthor | human hematopoietic cell line | - |
dc.subject.keywordAuthor | KG1a | - |
dc.subject.keywordAuthor | cell tracking | - |
dc.subject.keywordPlus | LYMPHOBLASTIC LEUKEMIC-CELLS | - |
dc.subject.keywordPlus | MARROW STROMAL CELLS | - |
dc.subject.keywordPlus | HAMSTER-CHEEK POUCH | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PLATELET-AGGREGATION | - |
dc.subject.keywordPlus | GROWTH-PARAMETERS | - |
dc.subject.keywordPlus | INVIVO TRACKING | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | ADHESION | - |
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