Electrocatalytic properties of metal-dispersed carbon paste electrode for reagentless L-lactate biosenso

Abstract

Carbon paste electrode를 채용함으로써 L-lac tate 측정용의 전기화학식 바이오센서를 성공적으로 개발할 수 있었다. 특히 뛰어난 electrocatalytic activity를 갖는 platinum이 첨가된 platinum-CPE 를 이용하여 낮은 전위에서의 NADH의 전기척 산 화가 가능하였다. Enzyme (lactate dehydrogen­a ase)과 NAD+를 carbon paste형식으로 직접 제조 함으로써 L-lactate 측정을 위한 성공적인 바이요센 서의 개발이 가능하였다. 위와 같은 개발연구를 통 하여 다른 NAD+ -dependent dehydrogenase를 채 용한 바이오센서로의 적용이 기대된다.

Metal dispersed carbon paste electrodes were fabricated, and their electrochemical properties were investigated. Among various metal dispersed carbons, platinum-dispersed carbon paste electrode showed most efficient electrocatalytic characteristics. The overpotential for the oxidation of NADH was significantly lowered in the platinum-dispersed carbon paste electrode, and catalytic current was also enhanced. Based on these electrocatalytic observations, L-lactate biosensor using L-lactate dehydrogenase was constructed to evaluate its performance in terms of sensitivity and stability.