DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, MT | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-03T05:05:20Z | - |
dc.date.available | 2013-03-03T05:05:20Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-05 | - |
dc.identifier.citation | BIOPROCESS ENGINEERING, v.22, no.5, pp.403 - 406 | - |
dc.identifier.issn | 0178-515X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77353 | - |
dc.description.abstract | The plaque assay has been widely used for titration of adenovirus (AdV). However, it takes usually 2-3 weeks, so this slow assay often impedes bioprocess development of large-scale AdV production. In this study, we developed a rapid AdV titration assay that can be done within a day. Further, unlike the plaque assay, this assay does not require a laborious serial dilution of samples. This rapid assay can be achieved by using green fluorescent protein (GFP) as a marker gene and flow cytometric analysis. It yields a good correlation between infectious titer of AdV harboring GFP and flow cytometric parameters such as average green fluorescence intensity or % of infected cells. Taken together, this rapid assay will facilitate bioprocess development for efficient large-scale AdV production. | - |
dc.language | English | - |
dc.publisher | SPRINGER VERLAG | - |
dc.title | Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis | - |
dc.type | Article | - |
dc.identifier.wosid | 000087188400006 | - |
dc.identifier.scopusid | 2-s2.0-2442631835 | - |
dc.type.rims | ART | - |
dc.citation.volume | 22 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 403 | - |
dc.citation.endingpage | 406 | - |
dc.citation.publicationname | BIOPROCESS ENGINEERING | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Park, MT | - |
dc.type.journalArticle | Article | - |
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