Effects of exogenous compatible solutes on growth of the hyperthermophilic archaeon Sulfolobus solfataricus

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Six known compatible solutes as well as twenty L-amino acids were individually added to a glucose minimal medium and their effects on the growth of Sulfolobus solfataricus (DSM 1617) were examined. Among the compatible solutes tested, putrescine, trehalose, and L-glutamate enhanced the growth of S. solfataricus. On the other hand, glycine betaine, choline, and L-proline show-ed little or no influence on cell growth. When cells were grown in the glucose medium supplemented with trehalose or L-glutamate, S. solfataricus preferentially utilized the compatible solute over glucose. The growth-enhancement effect of L-glutamate was also observed to be dependent on the glucose concentration in the medium: growth enhancement was higher when the concentration of glucose was low and gradually decreased with increasing glucose concentration. Interestingly, the effects of amino acids on cell growth differed markedly depending on the chemical nature of the amino acid added. While acidic amino acids-L-glutamate and L-aspartate-enhanced the growth rate, almost no growth was observed in the presence of glycine, L-leucine, L-valine, L-phenylalanine, L-threonine, L-methionine, or L-cysteine. Among all the low-molecular-weight solutes tested in this study, the growth-stimulation effect was most profound in the presence of L-glutamate. When S, solfataricus cells were grown in a glucose (1.0 g/l) medium supplemented with 3.0 g/l L-glutamate, the maximal cell density and growth rate were about 3.2- and 2.3-fold higher than those obtained without L-glutamate.
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Issue Date
2000
Language
English
Article Type
Article
Keywords

OSMOTIC ADAPTATION; GLYCINE BETAINE; BACTERIA; GENUS; ARCHAEBACTERIA; METHANOSARCINA; HALOTOLERANCE; ACCUMULATION; TEMPERATURE; RESPONSES

Citation

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.89, no.4, pp.318 - 322

ISSN
1389-1723
DOI
10.1016/S1389-1723(00)88952-5
URI
http://hdl.handle.net/10203/77306
Appears in Collection
MS-Journal Papers(저널논문)
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