DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임현수 | ko |
dc.contributor.author | 김정회 | ko |
dc.date.accessioned | 2013-03-03T04:37:35Z | - |
dc.date.available | 2013-03-03T04:37:35Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-01 | - |
dc.identifier.citation | 한국미생물·생명공학회지, v.23, no.5, pp.499 - 505 | - |
dc.identifier.issn | 1598-642X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77254 | - |
dc.description.abstract | Immobilization of anchorage-dependent animal cells was investigated using macroporous gelatin microcarriers developed in our laboratory. For the observation of the distribution of cells in macroporous beads, Vero-6 cells and CHO cells were cuttured and their distribution in macroporous beads was observed using a confocal microscope. In results, the final concentration of Vero-6 cells and CHO cells on macroporous beads was 2-3 times higher than that on commercial solid microcarriers (Cytodex-3). Also, macroporous microcarriers could hold cells in their macropores. Consequently, the pores protected cells against hydrodynamic shear. Based on Kolmogorov eddy length scale, the smaller eddies (80 $\mu $m) showed the detrimental effect on cells in macroporous beads as compared with 160 $\mu $m of eddies in conventional solid microcarriers. | - |
dc.language | Korean | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | 다공성 젤라틴 미립담체를 이용한 동물세포의 배양시 담체 내외부의 세포 분포 및 전단응력의 영향 | - |
dc.title.alternative | Cell distribution and shear effect on mammalian cell growth in macroporous gelatin microcarriers | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 499 | - |
dc.citation.endingpage | 505 | - |
dc.citation.publicationname | 한국미생물·생명공학회지 | - |
dc.contributor.localauthor | 김정회 | - |
dc.contributor.nonIdAuthor | 임현수 | - |
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