Recovery of poly(3-hydroxybutyrate) from coagulated Ralstonia eutropha using a chemical digestion method

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For economic recovery of poly(3-hydroxybutyrate) (PHB) from culture broths of Ralstonia eutropha containing PHB, Al-based and Fe-based coagulants were used in the pretreatment step. The coagulated cells were then separated by centrifugation, and PHB was extracted by chemical digestion with a sodium hypochlorite/chloroform dispersion solution. The practical upper limits of dosage were found to be 1,500 mg-Al/L and 1,000 mg-Fe/L, respectively, for Al- and Fe-based coagulants. When the harvested cells were treated with a 50% sodium hypochlorite/chloroform dispersion solution, PHB recovery and purity were 90-94% and 98-99%, respectively. The influence of the use of coagulants on the PHB recovery process was found to be insignificant. Despite the residual Al and Fe in the recovered PHB (less than 450 mg-Al/kg-PHB and 750 mg-Fe/kg-PHB, respectively), no detectable amounts of Al and Fe were leached from films made of the recovered PHB under acidic conditions. The use of Fe-based coagulants is less recommended because the Fe impurity can cause an unwanted colorization problem in the final product.
Publisher
AMER CHEMICAL SOC
Issue Date
2000-07
Language
English
Article Type
Article
Keywords

ALCALIGENES-EUTROPHUS

Citation

BIOTECHNOLOGY PROGRESS, v.16, no.4, pp.676 - 679

ISSN
8756-7938
URI
http://hdl.handle.net/10203/77179
Appears in Collection
CBE-Journal Papers(저널논문)
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