DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Park, IY | ko |
dc.contributor.author | Kim, MS | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2013-03-02T18:09:31Z | - |
dc.date.available | 2013-03-02T18:09:31Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-07 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.50, no.1, pp.113 - 118 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74842 | - |
dc.description.abstract | To produce xylobiose from xylan, high-level expression of an endoxylanase gene from Bacillus sp. was carried out in Bacillus subtilis DB104. A 1.62-kb SmaI DNA fragment, coding for an endoxylanase of Bacillus sp., was ligated into the Escherichia coli/B. subtilis shuttle vector pJH27 Delta 88, producing pJHKJ4, which was subsequently transformed into B. subtilis DB104. A maximum endoxylanase activity of 105 U/ml was obtained from the supernatant of B. subtilis DB104 harboring pJHKJ4. The endoxylanase was purified to homogeneity by ion-exchange chromatography and the production profile of xylooligosaccharides from xylan by the endoxylanase was examined by HPLC with a carbohydrate analysis column. Xylobiose was the major product from xylan at 40 degrees C and its proportion in the xylan hydrolyzates increased with the reaction time; at 12 h, over 60% of the reaction products was xylobiose. These results suggest that xylobiose, which has a stimulatory effect on the selective growth of the intestinal bacterium Bifidobacterium, can be mass-produced effectively by the endoxylanase of Bacillus sp. cloned in B. subtilis. | - |
dc.language | English | - |
dc.publisher | SPRINGER VERLAG | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | DEGRADATION | - |
dc.title | High-level expression of an endoxylanase gene from Bacillus sp. in Bacillus subtilis DB104 for the production of xylobiose from xylan | - |
dc.type | Article | - |
dc.identifier.wosid | 000075299500016 | - |
dc.identifier.scopusid | 2-s2.0-0031828230 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 113 | - |
dc.citation.endingpage | 118 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Park, IY | - |
dc.contributor.nonIdAuthor | Kim, MS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | DEGRADATION | - |
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