DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin Woo | ko |
dc.contributor.author | Won, Jungyeon | ko |
dc.contributor.author | Sohn, Seonghyang | ko |
dc.contributor.author | Joe, Cheol O | ko |
dc.date.accessioned | 2013-03-02T17:46:43Z | - |
dc.date.available | 2013-03-02T17:46:43Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-03 | - |
dc.identifier.citation | JOURNAL OF CELL SCIENCE, v.113, no.6, pp.955 - 961 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74779 | - |
dc.description.abstract | The role of the N-terminal cleavage product of poly(ADP-ribose) polymerase (PARP) on UV mediated apoptosis was investigated in cultured HeLa cells. Ultrastructural analysis of cells expressing caspase-resistant PARP (PARP(D214A)) revealed the typical features of necrosis following UV treatment. However, cells co-expressing PARP(D214A) With the N-terminal fragment of PARP containing the DNA-binding domain underwent apoptosis instead of necrosis. In this study, we have demonstrated that the DNA-binding activity of the N-terminal fragment of PARP is important for the execution of apoptosis. Point mutations were introduced in the DNA-hinding sites of the N-terminal fragment. Cells co-expressing PARP(D214A) With the mutated N-terminal fragments neither stimulated apoptosis nor prevented necrosis in response to UV irradiation. The present study proposes that the DNA-binding activity of the N-terminal fragment of PARP in UV treated cells prevents cellular ATP depletion, a mechanism by which necrotic cell death is triggered. | - |
dc.language | English | - |
dc.publisher | Company Of Biologists Ltd | - |
dc.subject | ADP-RIBOSYLATION | - |
dc.subject | CASPASE ACTIVITY | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CALF THYMUS | - |
dc.subject | NECROSIS | - |
dc.subject | INHIBITION | - |
dc.subject | TOPOISOMERASE | - |
dc.subject | STIMULATION | - |
dc.subject | PREVENTION | - |
dc.subject | SUBSTRATE | - |
dc.title | DNA-Binding activity of the N-terminal cleavage product of poly(ADP-ribose) polymerase is required for UV mediated apoptosis | - |
dc.type | Article | - |
dc.identifier.wosid | 000086312400007 | - |
dc.identifier.scopusid | 2-s2.0-0034111148 | - |
dc.type.rims | ART | - |
dc.citation.volume | 113 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 955 | - |
dc.citation.endingpage | 961 | - |
dc.citation.publicationname | JOURNAL OF CELL SCIENCE | - |
dc.contributor.localauthor | Kim, Jin Woo | - |
dc.contributor.localauthor | Joe, Cheol O | - |
dc.contributor.nonIdAuthor | Won, Jungyeon | - |
dc.contributor.nonIdAuthor | Sohn, Seonghyang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordAuthor | apopotis | - |
dc.subject.keywordAuthor | necrosis | - |
dc.subject.keywordAuthor | poly(ADP-ribose) | - |
dc.subject.keywordAuthor | polymerase | - |
dc.subject.keywordAuthor | ATP depletion | - |
dc.subject.keywordPlus | ADP-RIBOSYLATION | - |
dc.subject.keywordPlus | CASPASE ACTIVITY | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CALF THYMUS | - |
dc.subject.keywordPlus | NECROSIS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | TOPOISOMERASE | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | PREVENTION | - |
dc.subject.keywordPlus | SUBSTRATE | - |
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