DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Won Il | ko |
dc.date.accessioned | 2013-03-02T17:33:14Z | - |
dc.date.available | 2013-03-02T17:33:14Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997-06 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.7, no.3, pp.326 - 334 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74737 | - |
dc.description.abstract | Hammerhead ribozymes have been extensively used to inhibit the expression of cellular genes or viral genes mainly in the animal study, In this study, we designed a ribozyme targeting the conserved leader sequences of cucumber mosaic virus (CMV) RNA 1 and 2. The ribozyme, with asymmetric lengths of flanking complementary regions, cleaved a model substrate RNA efficiently at 26 degrees C as well as at 37 degrees C or 50 degrees C in vitro. And the ribozyme encoding sequence was introduced into tobacco plants and expressed with the CaMV 35S promoter and 3' NOS terminator in a monomeric type (pBIR1), tandemly repeated type (pBIR3), and cotranscriptionally combined type (pRokR) with 2.2 copies of I17N satellite RNA, Virus challenging experiments in F-1 plants of respective transformants with CMV-Y showed specific reductions of viral RNA 1 and 2 in comparison with RNA 3 or 4. Although young plants of a three-leaf-stage showed rather similar mild symptom attenuations in all constructions compared to CMV-Y inoculated wild type, fully grown plants showed a differential degree of resistance upon systemic infections of CMV-Y in pRokR, pBIR3 and pBIR1 transformed plants in a decreasing order. | - |
dc.publisher | Korean Soc Molecular & Cellular Biology | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | SATELLITE RNA | - |
dc.subject | GENOMIC RNA | - |
dc.subject | GENE | - |
dc.subject | REPLICATION | - |
dc.subject | REQUIREMENT | - |
dc.subject | EXPRESSION | - |
dc.subject | RESISTANCE | - |
dc.subject | INVIVO | - |
dc.title | Ribozyme mediated targeting of cucumber mosaic virus RNA 1 and 2 in transgenic tobacco plants | - |
dc.type | Article | - |
dc.identifier.wosid | A1997XG80200004 | - |
dc.identifier.scopusid | 2-s2.0-0031591448 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 326 | - |
dc.citation.endingpage | 334 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.localauthor | Chung, Won Il | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | SATELLITE RNA | - |
dc.subject.keywordPlus | GENOMIC RNA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | REQUIREMENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | INVIVO | - |
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