Increment of Efficiency in the Identification of Noble Genes by Colony Hybridization assay

Cited 3 time in webofscience Cited 0 time in scopus
  • Hit : 274
  • Download : 0
For the rapid identification of noble genes in a specific tissue by computer analysis from the cDNA sequences determined by single-pass cDNA sequencing, clone redundancy was one of the major obstacles. To facilitate the efficiency in identification of noble genes, it was necessary to reduce the number of clones to be sequenced by eliminating the redundant clones for a rapid analysis. In order to increase the probability of isolating noble sequences from the cDNA clones of human fetal liver tissue origin, colony hybridization assay was adopted and redundant clones were efficiently removed. Four cDNA clones highly redundant in the human fetal liver cDNA libraries including alpha-globin, gamma-globin, serum albumin and H19 RNA sequences were selected as the probes. Two hundreds and sixty two cDNA clones were randomly selected and tested with the probes for hybridization properties. The identity of each cDNA clone giving positive or negative signals in the hybridization assay was determined by DNA homology search with the nucleic acid databases. Among the 76 clones giving positive signals, 57 clones (75%) were found to be identical to the probe sequences and could be eliminated by colony hybridization assay before neucleotide sequencing.
Publisher
Academic Press
Issue Date
1998-02
Language
English
Article Type
Article
Keywords

EXPRESSED SEQUENCE TAGS; CONSTRUCTION; SUBTRACTION; SYSTEM; LIVER

Citation

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, v.44, no.2, pp.225 - 233

ISSN
1039-9712
URI
http://hdl.handle.net/10203/74256
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 3 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0