5 processing of RNA I in an Escherichia coli strain carrying the rnpA49 mutation

Abstract

RNA I, a negative controller of ColE1-type plasmid replication, is metabolized by several RNases in Escherichia coil. Two small derivatives of RNA I are accumulated at nonpermissive temperatures in an E. coil strain carrying the mpA49 mutation, a thermosensitive mutation in the mpA gene encoding the protein component of RNase P. A primer extension analysis was carried out to compare 5' processing of RNA I in the E, coli mpA49 cells at both permissive and nonpermissive temperatures. Derivatives of RNA I having different 5' ends were observed in the cells grown at permissive and nonpermissive temperatures. Some of the derivatives may be generated by the cleavage of RNase P.