Cross-species reactivity of a monoclonal antibody against glutathione S-transferase fusion protein of human beta 2-adrenergic receptor

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The purpose of the present study was to produce and characterize a monoclonal antibody against human beta 2-adrenergic receptor. Male BALB/c mice were immunized with glutathione S-transferase (GST) fusion protein of the C-terminal portion of the human beta 2-adrenergic receptor which was expressed in E.Coli. The immunized splenocytes were fused with myeloma SP2/0-Ag14 cells and the resulting monoclonal antibody was named as mAb beta C02. The monoclonal antibody beta C02 was determined as IgM subtype and then purified by anti-mouse IgM-agarose affinity chromatography. The results of ELISA, Western blot, and immunocytochemistry showed that mAb beta C02 recognized human beta 2-adrenergic receptor in the beta 2-adrenergic receptor-GST fusion protein and human epidermoid carcinoma cell line A431 with highly specific immunoreactivity. In addition, mAb beta C02 showed cross-species reactivity against beta-adrenergic receptor of hamster lung and rat brain as revealed by We stern blot and immunohistochemistry. The monoclonal antibody beta C02 may provide useful tools for the study of the beta-adrenergic receptor of human and other species including rats.
Publisher
Academic Press
Issue Date
1998
Language
English
Article Type
Article
Keywords

BETA-ADRENERGIC RECEPTORS; BETA-1-ADRENERGIC RECEPTOR; ANTIPEPTIDE ANTIBODIES; BACTERIAL EXPRESSION; ESCHERICHIA-COLI; A431 CELLS; RAT-BRAIN; PURIFICATION; LOCALIZATION; IDENTIFICATION

Citation

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, v.45, no.2, pp.215 - 225

ISSN
1039-9712
URI
http://hdl.handle.net/10203/74123
Appears in Collection
BS-Journal Papers(저널논문)
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