DC Field | Value | Language |
---|---|---|
dc.contributor.author | Stamm, Stefan | ko |
dc.contributor.author | Casper, Diana | ko |
dc.contributor.author | Hanson, Veneta | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-03-02T12:59:21Z | - |
dc.date.available | 2013-03-02T12:59:21Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-01 | - |
dc.identifier.citation | MOLECULAR BRAIN RESEARCH, v.64, no.1, pp.108 - 118 | - |
dc.identifier.issn | 0169-328X | - |
dc.identifier.uri | http://hdl.handle.net/10203/73629 | - |
dc.description.abstract | Clathrin light chain B (LCB) is a major component of clathrin coated vesicles, which are structures involved in intracellular transport. A neuron-specific isoform of LCB is generated by incorporation of a single exon (EN) using an alternative splicing mechanism that reflects the special demands of neurons, such as axonal transport and synaptic neurotransmission. Here, we demonstrate that this 1 neuron-specific exon is developmentally regulated and is excluded in non-neuronal cells because its 5' and 3' splice sites deviate from the mammalian consensus sequences. A gel retardation assay indicated the presence of a developmentally regulated factor in brain that binds to the neuronal exon. In addition, EN usage is repressed by increasing the concentration of htra2-betal, a splice factor whose isoform expression is influenced by neuronal activity. We propose that a brain-specific factor is involved in EN recognition during development and adulthood. In addition, ubiquitously expressed splicing factors such as htra2-betal are involved in regulating EN expression in the adult brain. (C) 1999 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PRE-MESSENGER-RNA | - |
dc.subject | TRACT BINDING-PROTEIN | - |
dc.subject | SPLICING FACTORS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | NMDA RECEPTOR | - |
dc.subject | IN-VIVO | - |
dc.subject | CELL | - |
dc.subject | BRAIN | - |
dc.subject | DROSOPHILA | - |
dc.subject | INTRON | - |
dc.title | Regulation of the neuron-specific exon of clathrin light chain B | - |
dc.type | Article | - |
dc.identifier.wosid | 000078196900013 | - |
dc.identifier.scopusid | 2-s2.0-0033593649 | - |
dc.type.rims | ART | - |
dc.citation.volume | 64 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 108 | - |
dc.citation.endingpage | 118 | - |
dc.citation.publicationname | MOLECULAR BRAIN RESEARCH | - |
dc.identifier.doi | 10.1016/S0169-328X(98)00313-1 | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Stamm, Stefan | - |
dc.contributor.nonIdAuthor | Casper, Diana | - |
dc.contributor.nonIdAuthor | Hanson, Veneta | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | alternative splicing | - |
dc.subject.keywordAuthor | clathrin light chain B | - |
dc.subject.keywordAuthor | transformer-2 | - |
dc.subject.keywordPlus | PRE-MESSENGER-RNA | - |
dc.subject.keywordPlus | TRACT BINDING-PROTEIN | - |
dc.subject.keywordPlus | SPLICING FACTORS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | NMDA RECEPTOR | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | DROSOPHILA | - |
dc.subject.keywordPlus | INTRON | - |
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