DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, WY | ko |
dc.contributor.author | Lee, KS | ko |
dc.contributor.author | Kim, TH | ko |
dc.contributor.author | Shin, MC | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2013-02-28T06:52:51Z | - |
dc.date.available | 2013-02-28T06:52:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-01 | - |
dc.identifier.citation | ELECTROANALYSIS, v.12, no.1, pp.78 - 82 | - |
dc.identifier.issn | 1040-0397 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73350 | - |
dc.description.abstract | A conductometric biosensor nas constructed with a sol-gel immobilized urease on a microfabricated interdigitated array (IDA) gold electrode. Tetramethoxysilane (TMOS) was used as a sol-gel precursor for the immobilization of the urease and the performance of the resulting urea biosensor was controlled through the water content in the acid-catalyzed hydrolysis of the sol-gel stock solution. The sol-gel-derived urea biosensor showed relatively wide dynamic range of 0.2-50 mM in 5 mM imidazole-HCl buffer at pH 7.5. Furthermore, the urea biosensor exhibited good sensor-to-sensor reproducibility (6.5 %) and storage stability (50 % of its original activity retained after 3 weeks) when stored in 5 mM imidazole-HCl buffer at 4 degrees C. The urea biosensor was successfully applied for the urea determination in urine sample by employing the differential measurement format consisting of an active IDA conductometric sensor containing sol-gel-immobilized urease and a reference IDA conductometric sensor containing bovine serum albumin layer instead of the urease. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | AMPEROMETRIC BIOSENSORS | - |
dc.subject | HYDROGEN-PEROXIDE | - |
dc.subject | GLUCOSE BIOSENSOR | - |
dc.subject | ENZYME | - |
dc.subject | CARBON | - |
dc.subject | TRANSDUCER | - |
dc.title | Microfabricated conductometric urea biosensor based on sol-gel immobilized urease | - |
dc.type | Article | - |
dc.identifier.wosid | 000084759600014 | - |
dc.identifier.scopusid | 2-s2.0-20644466652 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 78 | - |
dc.citation.endingpage | 82 | - |
dc.citation.publicationname | ELECTROANALYSIS | - |
dc.identifier.doi | 10.1002/(SICI)1521-4109(20000101)12:1<78::AID-ELAN78>3.3.CO;2-2 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.nonIdAuthor | Lee, WY | - |
dc.contributor.nonIdAuthor | Lee, KS | - |
dc.contributor.nonIdAuthor | Kim, TH | - |
dc.contributor.nonIdAuthor | Shin, MC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | conductometric | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | sol-gel immobilization | - |
dc.subject.keywordAuthor | urea | - |
dc.subject.keywordAuthor | urease | - |
dc.subject.keywordPlus | AMPEROMETRIC BIOSENSORS | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | GLUCOSE BIOSENSOR | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | CARBON | - |
dc.subject.keywordPlus | TRANSDUCER | - |
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