DC Field | Value | Language |
---|---|---|
dc.contributor.author | H. G. Jeong | ko |
dc.contributor.author | C. H. Yun | ko |
dc.contributor.author | Y. J. Jeon | ko |
dc.contributor.author | S. S. Lee | ko |
dc.contributor.author | Yang, Kyu Hwan | ko |
dc.date.accessioned | 2013-02-28T05:33:28Z | - |
dc.date.available | 2013-02-28T05:33:28Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-03 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.208, no.3, pp.1124 - 1130 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/73025 | - |
dc.description.abstract | Cultured mouse hepatoma cell line Hepa-1c1c7 cells were treated with methoxsalen to assess the role of methoxsalen in the process of Cyp1a-1 induction. Treatment of Hepa-1c1c7 cultures with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced Cyp1a-1, as indicated by analysis of 7-ethoxyresorufin O-deethylation (EROD) activity and P4501A1 protein. When methoxsalen and TCDD were both added to cultures, TCDD-inducible EROD activity was greatly suppressed by methoxsalen in a dose-dependent manner. We find that treatment of Hepa-1c1c7 cells with methoxsalen inhibited CYP1A1 mRNA induction by TCDD as well as the concomitant increase P4501A1 protein. Formation of DNA-protein complexes between the dioxin receptor and its DRE target was inhibited by methoxsalen, as determined by gel mobility shift assays using oligonucleotides corresponding to DRE 3 of the Cyp1a-1 gene. These results suggest that the inhibitory action of methoxsalen on TCDD induction of the Cyp1a-1 gene expression in Hepa-1c1c7 cells might be antagonism of the DNA binding potential of nuclear dioxin receptor. (C) 1995 Academic Press, Inc. | - |
dc.language | English | - |
dc.publisher | Academic Press Inc Elsevier Science | - |
dc.subject | PROTEIN-KINASE-C | - |
dc.subject | PSORALEN DERIVATIVES | - |
dc.subject | DRUG METHOXSALEN | - |
dc.subject | AH RECEPTOR | - |
dc.subject | BINDING | - |
dc.subject | 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN | - |
dc.subject | INACTIVATION | - |
dc.subject | LIVER | - |
dc.subject | GENE | - |
dc.title | SUPPRESSION OF CYTOCHROME-P450 (CYPLA-1) INDUCTION IN MOUSE HEPATOMA HEPA-1C1C7 CELLS BY METHOXSALEN | - |
dc.type | Article | - |
dc.identifier.wosid | A1995QP67700032 | - |
dc.identifier.scopusid | 2-s2.0-0028963098 | - |
dc.type.rims | ART | - |
dc.citation.volume | 208 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1124 | - |
dc.citation.endingpage | 1130 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.nonIdAuthor | H. G. Jeong | - |
dc.contributor.nonIdAuthor | C. H. Yun | - |
dc.contributor.nonIdAuthor | Y. J. Jeon | - |
dc.contributor.nonIdAuthor | S. S. Lee | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | PSORALEN DERIVATIVES | - |
dc.subject.keywordPlus | DRUG METHOXSALEN | - |
dc.subject.keywordPlus | AH RECEPTOR | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | GENE | - |
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