DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hoe, KL | ko |
dc.contributor.author | Won, MS | ko |
dc.contributor.author | Chung, KS | ko |
dc.contributor.author | Park, SK | ko |
dc.contributor.author | Kim, DU | ko |
dc.contributor.author | Jang, YJ | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | Yoo, HS | ko |
dc.date.accessioned | 2013-02-28T04:52:25Z | - |
dc.date.available | 2013-02-28T04:52:25Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-07 | - |
dc.identifier.citation | GENE, v.215, no.2, pp.319 - 328 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.uri | http://hdl.handle.net/10203/72864 | - |
dc.description.abstract | As a first step to elucidate the functions of Schizosaccharomyces pombe (S. pombe) GATA factors, we have isolated the gaf1(+) gene (GATA-factor like gene) in S. pombe. The predicted amino acid (aa) sequence of Gaf1 reveals a single zinc finger domain typical of fungal GATA factors, and the zinc finger exhibits 60% aa identity to that of human GATA-1. The open reading frame of Gaf1 predicts a protein of M-r 32 kDa consisting of 290 intronless amino acids. Disruption of this gene has no effect on cell viability and growth rate. The GST-Gaf1 fusion protein binds specifically to GATA motifs of its own promoter as well as DAL7 UAS, a canonical GATA motif of Saccharomyces cerevisiae (S. cerevisiae) The specific DNA-binding activity resides within the N-terminal half of Gaf1 (Gaf1N; aa 1-120) containing the zinc finger, whereas the C-terminal half (Gaf1C; aa 121-290) contains transactivation sequences that induce the expression of the lacZ reporter when fused to the GAL4 DNA binding domain. These results demonstrate that Gaf1 may function as a transcriptional activator consisting of DNA-binding and transactivation domains. (C) 1998 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PUTATIVE ZINC FINGER | - |
dc.subject | DNA-BINDING DOMAIN | - |
dc.subject | NITROGEN CATABOLITE REPRESSION | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | REGULATORY GENE | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEIN | - |
dc.subject | CELLS | - |
dc.subject | SPECIFICITY | - |
dc.subject | SEQUENCES | - |
dc.title | Molecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator | - |
dc.type | Article | - |
dc.identifier.wosid | 000075577500011 | - |
dc.identifier.scopusid | 2-s2.0-0032581725 | - |
dc.type.rims | ART | - |
dc.citation.volume | 215 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 319 | - |
dc.citation.endingpage | 328 | - |
dc.citation.publicationname | GENE | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Hoe, KL | - |
dc.contributor.nonIdAuthor | Won, MS | - |
dc.contributor.nonIdAuthor | Chung, KS | - |
dc.contributor.nonIdAuthor | Park, SK | - |
dc.contributor.nonIdAuthor | Kim, DU | - |
dc.contributor.nonIdAuthor | Jang, YJ | - |
dc.contributor.nonIdAuthor | Yoo, HS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | zinc finger | - |
dc.subject.keywordAuthor | GATA-binding | - |
dc.subject.keywordAuthor | transcription factor | - |
dc.subject.keywordAuthor | degenerate PCR | - |
dc.subject.keywordAuthor | S-pombe | - |
dc.subject.keywordPlus | PUTATIVE ZINC FINGER | - |
dc.subject.keywordPlus | DNA-BINDING DOMAIN | - |
dc.subject.keywordPlus | NITROGEN CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | REGULATORY GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | SEQUENCES | - |
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