Frequent occurrence of transgene deletion in transgenic plants

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The status of the transgene in tobacco plants transformed by Agrobacterium was analyzed with PCR, Twelve percent of the transgenic plants with the nptII gene showed different levels of transgene deletion, which was also found in transgenic watermelon (10-30%) and carrot (40-60%), It appeared that the percentage of transgenic plants carrying deleted transgenes depended on both the transgene and the plant. It is suggested that the transgene should be inserted between a right border and a selection marker to reduce the number of transgenic plants containing deleted transgenes after selection.
Publisher
SPRINGER-VERLAG SINGAPORE PTE LTD
Issue Date
1998-12
Language
English
Article Type
Article
Keywords

T-DNA INTEGRATION; AGROBACTERIUM-TUMEFACIENS; ILLEGITIMATE RECOMBINATION; TRANSFORMATION; GENOME

Citation

MOLECULES AND CELLS, v.8, no.6, pp.705 - 708

ISSN
1016-8478
URI
http://hdl.handle.net/10203/72540
Appears in Collection
MSE-Journal Papers(저널논문)
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