DC Field | Value | Language |
---|---|---|
dc.contributor.author | dong eun chang | ko |
dc.contributor.author | heung chae chung | ko |
dc.contributor.author | Rhee, Joon Shick | ko |
dc.contributor.author | jae gu pan | ko |
dc.date.accessioned | 2013-02-28T02:33:50Z | - |
dc.date.available | 2013-02-28T02:33:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-04 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.65, no.4, pp.1384 - 1389 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/72315 | - |
dc.description.abstract | We investigated metabolic engineering of fermentation pathways in Escherichia coli for production of optically pure D- or L-lactate, Several pta mutant strains were examined, and a pta mutant of E. coli RR1 which was deficient in the phosphotransacetylase of the Pta-AckA pathway was found to metabolize glucose to D-lactate and to produce a small amount of succinate by-product under anaerobic conditions. An additional mutation in ppc made the mutant produce D-lactate like a homofermentative lactic acid bacterium. When the pta ppc double mutant was grown to higher biomass concentrations under aerobic conditions before it shifted to the anaerobic phase of D-lactate production, more than 62.2 g of D-lactate per liter was produced in 60 h, and the volumetric productivity was 1.04 g/liter/h. To examine whether the blocked acetate flux could be reoriented to a nonindigenous L-lactate pathway, an L-lactate dehydrogenase gene from Lactobacillus casei was introduced into a pta ldhA strain which lacked phosphotransacetylase and D-lactate dehydrogenase. This recombinant strain was able to metabolize glucose to L-lactate as the major fermentation product, and up to 45 g of L-lactate per liter was produced in 67 h, These results demonstrate that the central fermentation metabolism of E. coli can be reoriented to the production of D-lactate, an indigenous fermentation product, or to the production of L-lactate, a nonindigenous fermentation product. | - |
dc.language | English | - |
dc.publisher | Amer Soc Microbiology | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | DEHYDROGENASE GENE | - |
dc.subject | FERMENTATION | - |
dc.subject | MUTANTS | - |
dc.subject | DERIVATIVES | - |
dc.subject | EXPRESSION | - |
dc.subject | CLONING | - |
dc.subject | LACKING | - |
dc.title | Homofermentative production of D- or L-lactate in metabolically engineered Escherichia coli RR1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000079530000004 | - |
dc.identifier.scopusid | 2-s2.0-0032912881 | - |
dc.type.rims | ART | - |
dc.citation.volume | 65 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 1384 | - |
dc.citation.endingpage | 1389 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.contributor.nonIdAuthor | dong eun chang | - |
dc.contributor.nonIdAuthor | heung chae chung | - |
dc.contributor.nonIdAuthor | jae gu pan | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | DEHYDROGENASE GENE | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | LACKING | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.