Purification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2

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dc.contributor.authorPark, J.H.ko
dc.contributor.authorKim, G.J.ko
dc.contributor.authorLee, S.G.ko
dc.contributor.authorLee, D.C.ko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2013-02-27T23:19:33Z-
dc.date.available2013-02-27T23:19:33Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-01-
dc.identifier.citationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.81, no.1, pp.53 - 65-
dc.identifier.issn0273-2289-
dc.identifier.urihttp://hdl.handle.net/10203/71449-
dc.description.abstractA thermostable D-hydantoinase was isolated from thermophilic Bacillus thermoatenalatus GH-2 and purified to homogeneity by using immunoaffinity chromatography. The molecular mass of the enzyme was determined to be about 230 kDa, and a value of 56 kDa was obtained as a molecular mass of the subunit on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, implying that oligomeric structure of the enzyme is tetrameric. Isoelectric pH of the enzyme was found to be approx 4.3. The enzyme required Mn2+ for the activity and exhibited its highest activity with phenylhydantoin as a substrate. The optimal pH and temperature for catalytic activity were about 7.5 and 65 degrees C, respectively. The half-life of the enzyme was estimated to be about 45 min at 80 degrees C.-
dc.languageEnglish-
dc.publisherHUMANA PRESS INC-
dc.subjectMICROBIAL TRANSFORMATION-
dc.subjectAMINO-ACIDS-
dc.subjectSTEAROTHERMOPHILUS SD-1-
dc.subjectENZYME-
dc.subjectMICROORGANISMS-
dc.subjectEXPRESSION-
dc.subjectCLONING-
dc.subjectGENE-
dc.titlePurification and characterization of thermostable D-hydantoinase from Bacillus thermocatenulatus GH-2-
dc.typeArticle-
dc.identifier.wosid000082748700005-
dc.identifier.scopusid2-s2.0-0032873779-
dc.type.rimsART-
dc.citation.volume81-
dc.citation.issue1-
dc.citation.beginningpage53-
dc.citation.endingpage65-
dc.citation.publicationnameAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.nonIdAuthorPark, J.H.-
dc.contributor.nonIdAuthorKim, G.J.-
dc.contributor.nonIdAuthorLee, S.G.-
dc.contributor.nonIdAuthorLee, D.C.-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorthermostability-
dc.subject.keywordAuthorD-hydantoinase-
dc.subject.keywordAuthorBacillus-
dc.subject.keywordAuthorimmunoaffinity-
dc.subject.keywordPlusMICROBIAL TRANSFORMATION-
dc.subject.keywordPlusAMINO-ACIDS-
dc.subject.keywordPlusSTEAROTHERMOPHILUS SD-1-
dc.subject.keywordPlusENZYME-
dc.subject.keywordPlusMICROORGANISMS-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusCLONING-
dc.subject.keywordPlusGENE-
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