DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, SJ | ko |
dc.contributor.author | Kang, Kae Won | ko |
dc.date.accessioned | 2013-02-27T23:11:29Z | - |
dc.date.available | 2013-02-27T23:11:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-07 | - |
dc.identifier.citation | PROTEIN EXPRESSION AND PURIFICATION, v.16, no.2, pp.340 - 346 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | http://hdl.handle.net/10203/71407 | - |
dc.description.abstract | A cysteine-rich (approximately 20%), low molecular weight (MW 6 kDa) polypeptide has been isolated from the Korean blood-sucking leech, Hirudo nipponia. From its amino acid composition and N-terminal amino acid sequence analysis, the new protein is similar to granulin (or epithelin), and so it has been named leech granulin. The leech granulin behaved as a thrombin inhibitor in the purification steps of size-exclusion, ion-exchange, chromatofocusing, and reverse-phase high-performance liquid chromatography. The leech granulin is an acidic peptide (pl 3.75) containing high cysteine residues with a unique sequence similar to granulins or epithelins isolated from other organisms. For the first time, a granulin-like polypeptide having thrombin inhibitory activity has been isolated from a leech species. (C) 1999 Academic Press. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC | - |
dc.subject | EPITHELIN PRECURSOR | - |
dc.subject | GROWTH-FACTORS | - |
dc.subject | PROTEINS | - |
dc.subject | PEPTIDE | - |
dc.subject | SEQUENCE | - |
dc.subject | GENE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | GIBBERELLIN | - |
dc.subject | EXPRESSION | - |
dc.subject | FAMILY | - |
dc.title | Purification of granulin-like polypeptide from the blood-sucking leech, Hirudo nipponia | - |
dc.type | Article | - |
dc.identifier.wosid | 000081421300015 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 340 | - |
dc.citation.endingpage | 346 | - |
dc.citation.publicationname | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.contributor.nonIdAuthor | Hong, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | EPITHELIN PRECURSOR | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | GIBBERELLIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FAMILY | - |
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