DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rim, SB | ko |
dc.contributor.author | Cho, B | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.contributor.author | Park, I | ko |
dc.date.accessioned | 2013-02-27T22:49:58Z | - |
dc.date.available | 2013-02-27T22:49:58Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-03 | - |
dc.identifier.citation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.29, no.2, pp.133 - 136 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | http://hdl.handle.net/10203/71298 | - |
dc.description.abstract | The affinity cleavage reagent methidiumpropyl-EDTA-Iron(II) is applied to the structural analysis of 5S rRNA. Analysis of cleavage sites induced by MPE-Fe(II) on 5S rRNA shows that MPE intercalates easily between the unstable base pairs or into the bulges, thereby ii strongly cuts the nucleosides nearby. The stable helical stems A, B, D and E as well as loop d are weakly cut. Most of the single-stranded loops are not cleaved. Based on the cleavage pattern of the 5S rRNA by MPE-Fe(II) and RNase V1, we suggest that MPE-Fe(II) may be used as a potential chemical probe in searching for the unstable helical regions of RNA, and for the sequences that appear to be involved in folding and distorting 5S rRNA. | - |
dc.language | English | - |
dc.publisher | BIOCHEMICAL SOC REPUBLIC KOREA | - |
dc.subject | DNA | - |
dc.title | Detection of cleavage sites on methidiumpropyl-EDTA-iron(II) | - |
dc.type | Article | - |
dc.identifier.wosid | A1996UC46000007 | - |
dc.type.rims | ART | - |
dc.citation.volume | 29 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 133 | - |
dc.citation.endingpage | 136 | - |
dc.citation.publicationname | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Rim, SB | - |
dc.contributor.nonIdAuthor | Cho, B | - |
dc.contributor.nonIdAuthor | Park, I | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | chemical nuclease | - |
dc.subject.keywordAuthor | methidiumpropyl-EDTA | - |
dc.subject.keywordAuthor | 5S rRNA | - |
dc.subject.keywordPlus | DNA | - |
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