DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang Y.K. | ko |
dc.contributor.author | Lee C.S.. | ko |
dc.contributor.author | Lee K.K. | ko |
dc.contributor.author | Chung, An Sik | ko |
dc.date.accessioned | 2013-02-27T22:05:34Z | - |
dc.date.available | 2013-02-27T22:05:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.8, no.3, pp.259 - 265 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/71106 | - |
dc.description.abstract | We have analyzed the regulatory roles of the first intron (intron-1) of the bovine beta-casein gene in the bovine beta-casein/CAT expression system using a mouse mammary epithelial cell line, HC11, After a combined treatment of HC11 cells with insulin, dexamethasone, and prolactin, the induced expression of p beta c1.8/+ICAT vector including 2 kb intron-1 and 1.8 kb promoter was greatly increased to 23.5 folds, while that of p beta c1.8CAT basic vector with 1.8 kb promoter only, was 6.5, A classical enhancer activity was shown in the 2 kb intron fragment from the experiment in which the orientation and the position of the intron-1 on the vectors were changed, The enhancer activity was largely dependent on the lactogenic hormones, especially prolactin, A stepwise reduction of the inducibility in the 5' to 3' deletion analysis of the intron-1 indicates the existence of several functional elements in the region. In particular, an internal fragment (+1071 to +1490) was important for the prolactin-dependent enhancing activity of the intron-1, These results suggest that several elements in the intron-1 of the bovine beta-casein gene cooperatively interact not only with each other but also with its promoter for hormonal induction. | - |
dc.language | English | - |
dc.publisher | Korean Soc Molecular & Cellular Biology | - |
dc.subject | TISSUE-SPECIFIC EXPRESSION | - |
dc.subject | EPITHELIAL-CELL LINE | - |
dc.subject | TRANSGENIC MICE | - |
dc.subject | MAMMARY-GLAND | - |
dc.subject | NUCLEAR FACTOR | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | PROMOTER | - |
dc.subject | TRANSCRIPTION | - |
dc.subject | HORMONES | - |
dc.subject | REPRESSION | - |
dc.title | Prolactin-inducible Enhancer Activity of the First Intron of the Bovine beta-Casein Gene | - |
dc.type | Article | - |
dc.identifier.wosid | 000074788100002 | - |
dc.identifier.scopusid | 2-s2.0-0032580834 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 259 | - |
dc.citation.endingpage | 265 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.nonIdAuthor | Kang Y.K. | - |
dc.contributor.nonIdAuthor | Lee C.S.. | - |
dc.contributor.nonIdAuthor | Lee K.K. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | beta-casein | - |
dc.subject.keywordAuthor | enhancer | - |
dc.subject.keywordAuthor | HC11 cell | - |
dc.subject.keywordAuthor | intron-1 | - |
dc.subject.keywordAuthor | prolactin | - |
dc.subject.keywordPlus | TISSUE-SPECIFIC EXPRESSION | - |
dc.subject.keywordPlus | EPITHELIAL-CELL LINE | - |
dc.subject.keywordPlus | TRANSGENIC MICE | - |
dc.subject.keywordPlus | MAMMARY-GLAND | - |
dc.subject.keywordPlus | NUCLEAR FACTOR | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | HORMONES | - |
dc.subject.keywordPlus | REPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.