Prolactin-inducible Enhancer Activity of the First Intron of the Bovine beta-Casein Gene

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dc.contributor.authorKang Y.K.ko
dc.contributor.authorLee C.S..ko
dc.contributor.authorLee K.K.ko
dc.contributor.authorChung, An Sikko
dc.date.accessioned2013-02-27T22:05:34Z-
dc.date.available2013-02-27T22:05:34Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1998-
dc.identifier.citationMOLECULES AND CELLS, v.8, no.3, pp.259 - 265-
dc.identifier.issn1016-8478-
dc.identifier.urihttp://hdl.handle.net/10203/71106-
dc.description.abstractWe have analyzed the regulatory roles of the first intron (intron-1) of the bovine beta-casein gene in the bovine beta-casein/CAT expression system using a mouse mammary epithelial cell line, HC11, After a combined treatment of HC11 cells with insulin, dexamethasone, and prolactin, the induced expression of p beta c1.8/+ICAT vector including 2 kb intron-1 and 1.8 kb promoter was greatly increased to 23.5 folds, while that of p beta c1.8CAT basic vector with 1.8 kb promoter only, was 6.5, A classical enhancer activity was shown in the 2 kb intron fragment from the experiment in which the orientation and the position of the intron-1 on the vectors were changed, The enhancer activity was largely dependent on the lactogenic hormones, especially prolactin, A stepwise reduction of the inducibility in the 5' to 3' deletion analysis of the intron-1 indicates the existence of several functional elements in the region. In particular, an internal fragment (+1071 to +1490) was important for the prolactin-dependent enhancing activity of the intron-1, These results suggest that several elements in the intron-1 of the bovine beta-casein gene cooperatively interact not only with each other but also with its promoter for hormonal induction.-
dc.languageEnglish-
dc.publisherKorean Soc Molecular & Cellular Biology-
dc.subjectTISSUE-SPECIFIC EXPRESSION-
dc.subjectEPITHELIAL-CELL LINE-
dc.subjectTRANSGENIC MICE-
dc.subjectMAMMARY-GLAND-
dc.subjectNUCLEAR FACTOR-
dc.subjectMESSENGER-RNA-
dc.subjectPROMOTER-
dc.subjectTRANSCRIPTION-
dc.subjectHORMONES-
dc.subjectREPRESSION-
dc.titleProlactin-inducible Enhancer Activity of the First Intron of the Bovine beta-Casein Gene-
dc.typeArticle-
dc.identifier.wosid000074788100002-
dc.identifier.scopusid2-s2.0-0032580834-
dc.type.rimsART-
dc.citation.volume8-
dc.citation.issue3-
dc.citation.beginningpage259-
dc.citation.endingpage265-
dc.citation.publicationnameMOLECULES AND CELLS-
dc.contributor.nonIdAuthorKang Y.K.-
dc.contributor.nonIdAuthorLee C.S..-
dc.contributor.nonIdAuthorLee K.K.-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorbeta-casein-
dc.subject.keywordAuthorenhancer-
dc.subject.keywordAuthorHC11 cell-
dc.subject.keywordAuthorintron-1-
dc.subject.keywordAuthorprolactin-
dc.subject.keywordPlusTISSUE-SPECIFIC EXPRESSION-
dc.subject.keywordPlusEPITHELIAL-CELL LINE-
dc.subject.keywordPlusTRANSGENIC MICE-
dc.subject.keywordPlusMAMMARY-GLAND-
dc.subject.keywordPlusNUCLEAR FACTOR-
dc.subject.keywordPlusMESSENGER-RNA-
dc.subject.keywordPlusPROMOTER-
dc.subject.keywordPlusTRANSCRIPTION-
dc.subject.keywordPlusHORMONES-
dc.subject.keywordPlusREPRESSION-
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