Crystallization and preliminary X-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B

Abstract

The Delta(5)-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized, The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of a = 36.48 Angstrom, b = 74.30 Angstrom, c = 96.02 Angstrom, and contain one homodimer per asymmetric unit, Native diffraction data to 2.19 Angstrom resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement. (C) 1996 Wiley-Liss, Inc.