DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SW | ko |
dc.contributor.author | Kim, P | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2013-02-27T20:46:37Z | - |
dc.date.available | 2013-02-27T20:46:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-06 | - |
dc.identifier.citation | ENZYME AND MICROBIAL TECHNOLOGY, v.24, no.8-9, pp.555 - 560 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | http://hdl.handle.net/10203/70760 | - |
dc.description.abstract | Mass production of a copolymer consisting of S-hydroxybutyrate and 3-hydroxyvalerate, P(3HB-co-3HV), from Methylobacterium organophilum NCIB 11278 was investigated by using an automatic fed-batch culture technique. P(3HB-co-3HV) was produced when valeric acid was supplied as an auxiliary carbon source with methanol as the main carbon source. Among the sir nutrients tested (NH(4)(+), K(+), Mg(2)(+), SO(4)(2-), PO(4)(2-) and dissolved oxygen), the synthesis of P(3HB-co-3HV) was most favored in conditions of potassium limitation. The effect on copolymer production of the ratio of valeric acid to methanol (V/M ratio) in the feed was examined. At a V/M ratio of 1/10 (1 ml valeric acid per 10 ml methanol), a copolymer content of 41% of dry cell weight and a 3-hydroxyvaleric acid (3HV) fraction in the copolymer of 14 mol% were obtained. During 78 h of cultivation under these optimized conditions, accumulation of P(3HB-co-3HV) was as high as 99 g l(-1) with a 3HV fraction of 11.0 mol%. (C) 1999 Elsevier Science Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Methylobacterium organophilum by potassium-limited fed-batch culture | - |
dc.type | Article | - |
dc.identifier.wosid | 000080226700013 | - |
dc.type.rims | ART | - |
dc.citation.volume | 24 | - |
dc.citation.issue | 8-9 | - |
dc.citation.beginningpage | 555 | - |
dc.citation.endingpage | 560 | - |
dc.citation.publicationname | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.identifier.doi | 10.1016/S0141-0229(98)00160-4 | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Kim, SW | - |
dc.contributor.nonIdAuthor | Kim, P | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) potassium limitation | - |
dc.subject.keywordAuthor | mass production | - |
dc.subject.keywordAuthor | Methylobacterium organophilum | - |
dc.subject.keywordAuthor | methanol | - |
dc.subject.keywordPlus | BETA-HYDROXYBUTYRIC ACID | - |
dc.subject.keywordPlus | ALCALIGENES-EUTROPHUS | - |
dc.subject.keywordPlus | POLY(BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE) | - |
dc.subject.keywordPlus | POLYHYDROXYALKANOATES | - |
dc.subject.keywordPlus | COPOLYESTERS | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | GLUCOSE | - |
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