Phopholipase A2-catalyzed transesterification of phophatidylcholine containing nervonic acid in organic solvent

Abstract

The phospholipase A(2)-catalyzed transesterification of phosphatidylcholine (PC, 95%) with nervonic acid (NA, 95%) was successfully carried out in an organic solvent. The maximum yield after 48 h was 10.3% (w/w) at 50 degreesC with an initial water activity (a(w)) of 0.16, and a molar ratio of NA to PC of 20 in 5 ml ethyl acetate.