DC Field | Value | Language |
---|---|---|
dc.contributor.author | G. H. Yang | ko |
dc.contributor.author | S. S. Rhee | ko |
dc.contributor.author | H. K. Kim | ko |
dc.contributor.author | Yang, Kyu Hwan | ko |
dc.date.accessioned | 2013-02-27T15:50:29Z | - |
dc.date.available | 2013-02-27T15:50:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-07 | - |
dc.identifier.citation | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, v.45, no.2, pp.401 - 407 | - |
dc.identifier.issn | 1039-9712 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69439 | - |
dc.description.abstract | The upstream region of the gene coding for Clostridium botulinum type B (BoNT/B) neurotoxin was cloned and sequenced. There were two open reading frames, which were identified as a nontoxic-nonhemagglutinin component (ntnh/B) and a 22 kDa adjacent open reading frame (orf22/B). Deduced primary structure of ntnh/B showed that it was composed of 1,197 amino acid residues. Pairwise comparisons of the ntnh/B component with other botulinum toxin types showed high degree of homology to ntnh/A (82% identity). Northern blot analysis revealed that toxin gene could be transcribed alone or co-transcribed with the ntnh gene. The orf22/B gene encoding for 178 amino acids (M.W. 21.6 kDa) was located between the 33 kDa hemagglutinin gene and the ntnh gene. Orf22/B also showed high degree of homology to orf22/A (98.9% identity). These results suggested that the upstream region of the BoNT/B gene (containing the ntnh/B and orf22/B genes) might be evolutionarily closely related to the counterparts of the BoNT/A. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS AUST | - |
dc.subject | NONTOXIC-NONHEMAGGLUTININ COMPONENT | - |
dc.subject | COMPLETE NUCLEOTIDE-SEQUENCE | - |
dc.subject | DIFFERENT MOLECULAR SIZES | - |
dc.subject | PROGENITOR TOXIN | - |
dc.subject | ORAL TOXICITIES | - |
dc.title | Cloning and characterization of the upstream region of Clostridium botulinum type B neurotoxin gene | - |
dc.type | Article | - |
dc.identifier.wosid | 000074556000021 | - |
dc.identifier.scopusid | 2-s2.0-0345404167 | - |
dc.type.rims | ART | - |
dc.citation.volume | 45 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 401 | - |
dc.citation.endingpage | 407 | - |
dc.citation.publicationname | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | - |
dc.contributor.nonIdAuthor | G. H. Yang | - |
dc.contributor.nonIdAuthor | S. S. Rhee | - |
dc.contributor.nonIdAuthor | H. K. Kim | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NONTOXIC-NONHEMAGGLUTININ COMPONENT | - |
dc.subject.keywordPlus | COMPLETE NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | DIFFERENT MOLECULAR SIZES | - |
dc.subject.keywordPlus | PROGENITOR TOXIN | - |
dc.subject.keywordPlus | ORAL TOXICITIES | - |
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