DC Field | Value | Language |
---|---|---|
dc.contributor.author | h.i. jung | ko |
dc.contributor.author | i.c. shin | ko |
dc.contributor.author | y.m. park | ko |
dc.contributor.author | Kang, Kae Won | ko |
dc.contributor.author | k.s. ha | ko |
dc.date.accessioned | 2013-02-27T15:32:36Z | - |
dc.date.available | 2013-02-27T15:32:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.7, no.3, pp.431 - 437 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/69365 | - |
dc.description.abstract | Swiss 3T3 fibroblasts were treated with the microtubule-disrupting agent colchicine to study any interaction between microtubule dynamics and actin polymerization. Colchicine increased the amount of filamentous actin (F-actin), in a dose- and time-dependent manner with a significant increase at 1 h by about 130% over control level. Confocal microscopic observation showed that colchicine increased F-actin contents by stress fiber formation without inducing membrane ruffling. Colchicine did not activate phospholipase C and phospholipase D, whereas lysophosphatidic acid did, indicating that colchicine may have a different mechanism of actin polymerization regulation from LPA. A variety of microtubule-disrupting agents stimulated actin polymerization in Swiss 3T3 and Rat-2 fibroblasts as did colchicine, but the microtubule-stabilizing agent taxol inhibited actin polymerization induced by the above microtubule-disrupting agents. In addition, colchicine-induced actin polymerization was blocked by two protein phosphatase inhibitors, okadaic acid and calyculin A. These results suggest that microtubule depolymerization activates stress fiber formation by serine/threonine dephosphorylation in fibroblasts. | - |
dc.language | English | - |
dc.publisher | Korean Soc Molecular & Cellular Biology | - |
dc.subject | PROTEIN-KINASE-C | - |
dc.subject | TYROSINE-PHOSPHORYLATION | - |
dc.subject | LYSOPHOSPHATIDIC ACID | - |
dc.subject | PHOSPHATIDIC-ACID | - |
dc.subject | SIGNAL-TRANSDUCTION | - |
dc.subject | STRESS FIBERS | - |
dc.subject | DNA-SYNTHESIS | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | CELLS | - |
dc.subject | FIBROBLASTS | - |
dc.title | Cholchicine Activates Actin Polymerization by Microtabule Depolymerization | - |
dc.type | Article | - |
dc.identifier.wosid | A1997XG80200020 | - |
dc.identifier.scopusid | 2-s2.0-0031591544 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 431 | - |
dc.citation.endingpage | 437 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.nonIdAuthor | h.i. jung | - |
dc.contributor.nonIdAuthor | i.c. shin | - |
dc.contributor.nonIdAuthor | y.m. park | - |
dc.contributor.nonIdAuthor | k.s. ha | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | TYROSINE-PHOSPHORYLATION | - |
dc.subject.keywordPlus | LYSOPHOSPHATIDIC ACID | - |
dc.subject.keywordPlus | PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | STRESS FIBERS | - |
dc.subject.keywordPlus | DNA-SYNTHESIS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
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