DC Field | Value | Language |
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dc.contributor.author | Chung, Won Il | ko |
dc.date.accessioned | 2013-02-27T13:34:34Z | - |
dc.date.available | 2013-02-27T13:34:34Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-06 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, v.1489, no.2-3, pp.389 - 392 | - |
dc.identifier.issn | 0167-4781 | - |
dc.identifier.uri | http://hdl.handle.net/10203/68869 | - |
dc.description.abstract | The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A(2) (PLA(2)). Since little is known about PLA(2) gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca(2+) binding domain, a PLA(2) active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA(2). Amino acid sequence alignment of CFMI-3 to various putative plant PLA(2) confirmed that the CFMI-3 cDNA is the full-length putative PLA(2) cDNA identified in plant species. (C) 1999 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | Elsevier Science Bv | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | LEAVES | - |
dc.subject | ACIDS | - |
dc.title | Characterization of the full-length sequences of phospholipase A2 induced during flower development | - |
dc.type | Article | - |
dc.identifier.wosid | 000084528300017 | - |
dc.identifier.scopusid | 2-s2.0-0032753426 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1489 | - |
dc.citation.issue | 2-3 | - |
dc.citation.beginningpage | 389 | - |
dc.citation.endingpage | 392 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | - |
dc.contributor.localauthor | Chung, Won Il | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | carnation flower | - |
dc.subject.keywordAuthor | differential gene expression | - |
dc.subject.keywordAuthor | flower maturation | - |
dc.subject.keywordAuthor | phospholipase A(2) | - |
dc.subject.keywordAuthor | suppression subtractive hybridization | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | ACIDS | - |
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