DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, KJ | ko |
dc.contributor.author | Han, SH | ko |
dc.contributor.author | Kim, BY | ko |
dc.contributor.author | Hwang, SG | ko |
dc.contributor.author | Park, KK | ko |
dc.contributor.author | Yang, KH | ko |
dc.contributor.author | Chung, An Sik | ko |
dc.date.accessioned | 2013-02-27T13:27:37Z | - |
dc.date.available | 2013-02-27T13:27:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-07 | - |
dc.identifier.citation | TOXICOLOGY AND APPLIED PHARMACOLOGY, v.166, no.2, pp.120 - 127 | - |
dc.identifier.issn | 0041-008X | - |
dc.identifier.uri | http://hdl.handle.net/10203/68841 | - |
dc.description.abstract | Chlorophyllin (CHL), a water-soluble derivative of chlorophyll, functions as an anticarcinogen and antioxidant. In the present study, we investigated the effect of CHL on nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Treatment with CHL inhibited nitric oxide production in the LPS-stimulated RAW 264.7 cells in a dose-related manner. Competitive RT-PCR analysis, using a DNA competitor as an internal standard, demonstrated that the treatment with 1, 10, and 50 mu M CHL decreased LPS-induced iNOS mRNA expression in a concentration-dependent manner. Since the expression of the iNOS gene is mainly regulated by NF-kappa B, we then examined the effects of CHL on the NF-kappa B DNA binding activity, using an electrophoretic mobility shift assay. CHL down-regulated the NF-kappa B DNA binding on its cognate recognition site at the concentrations just noted. Employing a transfection and reporter gene expression system with p(NF-kappa B)(3)-chloramphenicol acetyl transferase (CAT), the treatment of CHL produced a dose-dependent inhibition of CAT activity in RAW 264.7 cells. Furthermore, CHL partially restored LPS-decreased I kappa B alpha, an inhibitory protein against NF-kappa B activation, in the cytosolic extract from the LPS-treated cells determined by immunoblot analysis. CHL also protected the hydroxyl radical-induced cytotoxicity in RAW 264.7 cells, indicating its antioxidant effect. These results suggest that CHL suppresses the nitric oxide production and iNOS mRNA expression mediated by the inhibition of NF-kappa B activation, and its action mechanism may be based on its antioxidant effect. (C) 2000 Academic Press. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC Elsevier Science | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | PERITONEAL-MACROPHAGES | - |
dc.subject | INHIBITS INDUCTION | - |
dc.subject | SYNTHASE GENE | - |
dc.subject | IN-VIVO | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | PEROXYNITRITE CAUSES | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | DNA-BINDING | - |
dc.title | Chlorophyllin suppression of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000088525700006 | - |
dc.type.rims | ART | - |
dc.citation.volume | 166 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 120 | - |
dc.citation.endingpage | 127 | - |
dc.citation.publicationname | TOXICOLOGY AND APPLIED PHARMACOLOGY | - |
dc.identifier.doi | 10.1006/taap.2000.8958 | - |
dc.contributor.nonIdAuthor | Cho, KJ | - |
dc.contributor.nonIdAuthor | Han, SH | - |
dc.contributor.nonIdAuthor | Kim, BY | - |
dc.contributor.nonIdAuthor | Hwang, SG | - |
dc.contributor.nonIdAuthor | Park, KK | - |
dc.contributor.nonIdAuthor | Yang, KH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | chlorophyllin | - |
dc.subject.keywordAuthor | nitric oxide | - |
dc.subject.keywordAuthor | lipopolysaccharide | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PERITONEAL-MACROPHAGES | - |
dc.subject.keywordPlus | INHIBITS INDUCTION | - |
dc.subject.keywordPlus | SYNTHASE GENE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | PEROXYNITRITE CAUSES | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | DNA-BINDING | - |
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