DC Field | Value | Language |
---|---|---|
dc.contributor.author | Geun-Joong Kim | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-02-27T11:39:37Z | - |
dc.date.available | 2013-02-27T11:39:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1998-01 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.243, no.1, pp.96 - 100 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/68330 | - |
dc.description.abstract | Most microbial D-hydantoinases have been reported to have catalytic properties similar to those of mammalian dihydropyrimidinases. Comparison of the primary structures of microbial D-hydantoinases with mammalian dihydropyrimidinases revealed that the amino acid homology is about 37% and functionally important residues are rigidly conserved at identical positions. Interestingly, however, the C-terminal regions mere found to be completely mismatched with each other. In order to investigate the possible role of the C-terminal regions, we deleted the C-terminal regions of the D-hydantoinases from two thermophilic Bacilli and compared the catalytic and structural properties of the mutant enzymes with those of wildtype enzymes. As a result, the C-terminal region was found not to be essential for catalysis, but it does affect the oligomeric structure of the enzyme. (C) 1998 Academic Press. | - |
dc.language | English | - |
dc.publisher | Academic Press Inc Elsevier Science | - |
dc.subject | AMINO-ACIDS | - |
dc.subject | MICROBIAL TRANSFORMATION | - |
dc.subject | MICROORGANISMS | - |
dc.subject | PURIFICATION | - |
dc.subject | CLONING | - |
dc.subject | ENZYME | - |
dc.subject | GENE | - |
dc.subject | DIHYDROPYRIMIDINASE | - |
dc.subject | AMIDOHYDROLASE | - |
dc.subject | EXPRESSION | - |
dc.title | C-Terminal Regions of D-Hydantoinases Are Nonessential for Catalysis, but Affect the Oligomeric Structure | - |
dc.type | Article | - |
dc.identifier.wosid | 000071862400018 | - |
dc.type.rims | ART | - |
dc.citation.volume | 243 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 96 | - |
dc.citation.endingpage | 100 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Geun-Joong Kim | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | MICROBIAL TRANSFORMATION | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DIHYDROPYRIMIDINASE | - |
dc.subject.keywordPlus | AMIDOHYDROLASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
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