DC Field | Value | Language |
---|---|---|
dc.contributor.author | GWACK, Y | ko |
dc.contributor.author | KIM, DW | ko |
dc.contributor.author | HAN, JH | ko |
dc.contributor.author | Choe, Joonho | ko |
dc.date.accessioned | 2013-02-27T10:13:11Z | - |
dc.date.available | 2013-02-27T10:13:11Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1995-04 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.5, no.2, pp.171 - 175 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67913 | - |
dc.description.abstract | The NS3 protein of hepatitis C virus is known to have a serine-type proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase activity based on the presence of sequence motifs, We demonstrated that the NS3 protein of hepatitis C virus expressed in insect cells possesses an NTPase activity, Employing a baculovirus expression system, we expressed the NS3 protein and partially purified it using a metal-binding chromatography, We found that two kinds of NS3 proteins were expressed in a baculovirus expression system, The p70 protein is an expected form and the p47 protein seems to be a proteolytic cleavage form of the p70 protein, The p70 protein showed a specific NTPase activity, NTPase activity was completely inhibited by NS3 monoclonal antibodies. Our data strongly suggest that the NS3 protein of HCV expressed in insect cells contains an NTPase activity. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR BIOLOGY | - |
dc.subject | NON-B-HEPATITIS | - |
dc.subject | RNA-STIMULATED NTPASE | - |
dc.subject | VIRAL DIARRHEA VIRUS | - |
dc.subject | NON-A | - |
dc.subject | POLYPROTEIN | - |
dc.subject | PESTIVIRUS | - |
dc.subject | GENOME | - |
dc.subject | FLAVIVIRUS | - |
dc.subject | CLEAVAGE | - |
dc.subject | DNA | - |
dc.title | NTPASE ACTIVITY OF HEPATITIS-C VIRUS NS3 PROTEIN EXPRESSED IN INSECT CELLS | - |
dc.type | Article | - |
dc.identifier.wosid | A1995QU74100013 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 171 | - |
dc.citation.endingpage | 175 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.localauthor | Choe, Joonho | - |
dc.contributor.nonIdAuthor | GWACK, Y | - |
dc.contributor.nonIdAuthor | KIM, DW | - |
dc.contributor.nonIdAuthor | HAN, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NON-B-HEPATITIS | - |
dc.subject.keywordPlus | RNA-STIMULATED NTPASE | - |
dc.subject.keywordPlus | VIRAL DIARRHEA VIRUS | - |
dc.subject.keywordPlus | NON-A | - |
dc.subject.keywordPlus | POLYPROTEIN | - |
dc.subject.keywordPlus | PESTIVIRUS | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | FLAVIVIRUS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | DNA | - |
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