DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이성택 | ko |
dc.date.accessioned | 2013-02-27T07:55:51Z | - |
dc.date.available | 2013-02-27T07:55:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1988 | - |
dc.identifier.citation | 산업미생물학회지, v.16, no.2, pp.79 - 84 | - |
dc.identifier.issn | 1598-642X | - |
dc.identifier.uri | http://hdl.handle.net/10203/67348 | - |
dc.description.abstract | β-Glucan 분해효소의 간편하며 예민한 활성도 측정방법으로 β-glucan에 색소와 cross linking agent를 접합시키는 변형기질 제조시 영향을 미치는 조건을 조사하고 맥아와 세균의 β-glucan분해효소 측정에 적응시켜 활성도를 측정한 결과는 다음과 같다. 1.0g β-glucan은 0.1N NaOH용액에서 색소 cibacron blue 3 G-A 1.5g과 cross linking agent인 1,4-butanedioldiglycidyl ether 1.25 ㎖을 90분간 끓여서 색소 접합기질로 제조하였을 때 β-glucanase 활성도 측정에 최적조건이었다. 변형기질은 pH5.3 에서 안정성을 보였으며 Bacillus subtilis K-4-3에서 추출한 효소액에 변형기질을 반응시켰을 때 간편하고 정확한 효소활성 측정이 가능하였다. 또한 색소방법을 DNS방법과 비교한 결과 색소방법이 β-glucan 분해효소 측정에 적당한 방법이었음이 입증되었다. Dye materials and cross linking agents were used for the determination of β-glucanase activities. The objective of this study was to prepare the blue coloured substrates which are sensitive, specific and simple for the determination of β-glucanase in malt and Bacillus subtilis K-4-3 enzymes. This method is based on the principle of measuring colorimetrically the split product of coloured and cross linked substrate. The best coupling of dye stuff of β-glucan was cibacron blue 3G-A and the colour released can suitably be measured at 623nm. Optimal concentration of dye and cross linking agents was 1.5g and 1.25㎖ under 0.1N NaOH. The sensitivity comparison proved that the stained β-glucan method is much more sensitive than the DNS method to determine reducing sugar released by the enzyme. | - |
dc.language | English | - |
dc.publisher | 산업미생물학회 | - |
dc.title | 맥아와 Bacillus subtilis B-4-3의 β-Glucan 분해 효소측정을 위한 새로운 색소기질 | - |
dc.title.alternative | A New Coloured Substrate for the Determination of β-Glucan Degrading Enzyme from Malt and Bacillus subtilis K-4-3 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 79 | - |
dc.citation.endingpage | 84 | - |
dc.citation.publicationname | 산업미생물학회지 | - |
dc.contributor.localauthor | 이성택 | - |
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