DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Chuck, AS | ko |
dc.contributor.author | Palsson, BO | ko |
dc.date.accessioned | 2013-02-27T07:49:29Z | - |
dc.date.available | 2013-02-27T07:49:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1993-04 | - |
dc.identifier.citation | BIOTECHNOLOGY TECHNIQUES, v.7, no.4, pp.271 - 276 | - |
dc.identifier.issn | 0951-208X | - |
dc.identifier.uri | http://hdl.handle.net/10203/67325 | - |
dc.description.abstract | In order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored at 4 and -20 °C, respectively. Flow cytometric analysis indicates that both fixation temperatures can be used in monitoring the changes in intracellular antibody content of the cells during a batch culture. However, it is better to fix and store the cells at -20 °C than 4 °C with regard to preservation of intracellular antibody and storage stability. © 1993 Science & Technology Letters. | - |
dc.language | English | - |
dc.publisher | Kluwer Academic Publishers | - |
dc.subject | SERUM-FREE MEDIA | - |
dc.subject | MONOCLONAL-ANTIBODIES | - |
dc.subject | CELL-LINE | - |
dc.subject | GROWTH | - |
dc.subject | PRODUCTIVITY | - |
dc.subject | ANTIGENS | - |
dc.subject | IGG | - |
dc.title | EFFECT OF FIXATION TEMPERATURE ON FLOW CYTOMETRIC MEASUREMENT OF INTRACELLULAR ANTIBODY CONTENT OF HYBRIDOMAS DURING BATCH CULTURE | - |
dc.type | Article | - |
dc.identifier.wosid | A1993KX71400005 | - |
dc.identifier.scopusid | 2-s2.0-0342716623 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 271 | - |
dc.citation.endingpage | 276 | - |
dc.citation.publicationname | BIOTECHNOLOGY TECHNIQUES | - |
dc.identifier.doi | 10.1007/BF00150897 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Chuck, AS | - |
dc.contributor.nonIdAuthor | Palsson, BO | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SERUM-FREE MEDIA | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | CELL-LINE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | PRODUCTIVITY | - |
dc.subject.keywordPlus | ANTIGENS | - |
dc.subject.keywordPlus | IGG | - |
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